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QUANTITATIVE FLUORESCENCE MICROSCOPY: From Art to Science

QUANTITATIVE FLUORESCENCE MICROSCOPY: From Art to Science Abstract A substantial number of elegant experimental approaches have been developed to image the distribution and dynamics of DNA, mRNA, proteins, organelles, metabolites, and ions in living plant cells. Although the human brain can rapidly assimilate visual information, particularly when presented as animations and movies, it is much more challenging to condense the phenomenal amount of data present in three-, four-, or even five-dimensional images into statistically useful measurements. This review explores a range of in vivo fluorescence imaging applications in plants, with particular emphasis on where quantitative techniques are beginning to emerge. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annual Review of Plant Biology Annual Reviews

QUANTITATIVE FLUORESCENCE MICROSCOPY: From Art to Science

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Publisher
Annual Reviews
Copyright
Copyright © 2006 by Annual Reviews. All rights reserved
ISSN
1040-2519
DOI
10.1146/annurev.arplant.57.032905.105239
pmid
16669756
Publisher site
See Article on Publisher Site

Abstract

Abstract A substantial number of elegant experimental approaches have been developed to image the distribution and dynamics of DNA, mRNA, proteins, organelles, metabolites, and ions in living plant cells. Although the human brain can rapidly assimilate visual information, particularly when presented as animations and movies, it is much more challenging to condense the phenomenal amount of data present in three-, four-, or even five-dimensional images into statistically useful measurements. This review explores a range of in vivo fluorescence imaging applications in plants, with particular emphasis on where quantitative techniques are beginning to emerge.

Journal

Annual Review of Plant BiologyAnnual Reviews

Published: Jun 2, 2006

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