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Volume changes induced by osmotic stress in freshly isolated rat hippocampal neurons

Volume changes induced by osmotic stress in freshly isolated rat hippocampal neurons  The degree to which osmotic stress changes the volume of mammalian central neurons has not previously been determined. We isolated CA1 pyramidal cells and measured cell volume in four different ways. Extracellular osmolarity (πo) was lowered by omitting varying amounts of NaCl and raised by adding mannitol; the extremes of πo tested ranged from 134 to 396 mosm/kg. When πo was reduced, cell swelling varied widely. We distinguished three types of cells according to their response: ”yielding cells” whose volume began to increase immediately; ”delayed response cells” which swelled after a latent period of 2 min or more; and ”resistant cells” whose volume did not change during exposure to hypo-osmotic solution. When πo was raised, most cells shrank slowly, reaching minimal volume in 15–20 min. We observed neither a regulatory volume decrease nor an increase. We conclude that the water permeability of the membrane of hippocampal CA1 pyramidal neurons is low compared to that of other cell types. The mechanical support of the plasma membrane given by the cytoskeleton may contribute to the resistance to swelling and protect neurons against swelling-induced damage. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Pflügers Archiv European Journal of Physiologyl of Physiology Springer Journals

Volume changes induced by osmotic stress in freshly isolated rat hippocampal neurons

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References (34)

Publisher
Springer Journals
Copyright
Copyright © 1998 by Springer-Verlag Berlin Heidelberg
Subject
Biomedicine; Human Physiology; Molecular Medicine; Neurosciences; Cell Biology; Receptors
ISSN
0031-6768
eISSN
1432-2013
DOI
10.1007/s004240050734
pmid
9799418
Publisher site
See Article on Publisher Site

Abstract

 The degree to which osmotic stress changes the volume of mammalian central neurons has not previously been determined. We isolated CA1 pyramidal cells and measured cell volume in four different ways. Extracellular osmolarity (πo) was lowered by omitting varying amounts of NaCl and raised by adding mannitol; the extremes of πo tested ranged from 134 to 396 mosm/kg. When πo was reduced, cell swelling varied widely. We distinguished three types of cells according to their response: ”yielding cells” whose volume began to increase immediately; ”delayed response cells” which swelled after a latent period of 2 min or more; and ”resistant cells” whose volume did not change during exposure to hypo-osmotic solution. When πo was raised, most cells shrank slowly, reaching minimal volume in 15–20 min. We observed neither a regulatory volume decrease nor an increase. We conclude that the water permeability of the membrane of hippocampal CA1 pyramidal neurons is low compared to that of other cell types. The mechanical support of the plasma membrane given by the cytoskeleton may contribute to the resistance to swelling and protect neurons against swelling-induced damage.

Journal

Pflügers Archiv European Journal of Physiologyl of Physiology – Springer Journals

Published: Oct 12, 1998

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