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References (31)
-
A. Coons, M. Kaplan (1949)
LOCALIZATION OF ANTIGEN IN TISSUE CELLSThe Journal of Experimental Medicine, 91
-
(1970)
Quantitative immunofluorescence. p. 6} in Holborow -
J. Ploem (1967)
The use of a vertical illuminator with interchangeable dichroic mirrors for fluorescence microscopy with incidental light.Zeitschrift fur wissenschaftliche Mikroskopie und mikroskopische Technik, 68 3
-
Bessis Bessis, Tabuis Tabuis (1955)
Aspect dynamique des plaquettes sanguines àľétat normal et pathologique. Analyse ?un film en contraste de phaseRev. Hémat., 10
-
C. Weerdt, J. Vreeken (1965)
Influence of Cryoprofibrin on the Antiglobulin Consumption Test with Platelets *Vox Sanguinis, 10
-
C. Engelfriet, K. Pondman, G. Wolters, A. Borne, D. Beckers, Gerda Misset-Groenveld, J. Loghem (1970)
Autoimmune haemolytic anaemias. 3. Preparation and examination of specific antisera against complement components and products, and their use in serological studies.Clinical and experimental immunology, 6 5
-
(1970)
ed.) Standardization in I IU tnnnoftuorescence -
D. Killander, A. Levin, M. Inoue, E. Klein (1970)
Quantification of immunofluorescence on individual erythrocytes coated with varying amounts of antigen.Immunology, 19 1
-
Performance testing of fluorescent antisera. p. 193 in Holborow. I -
J. Veen, T. Feltkamp (1971)
STUDIES ON THE STANDARDIZATION OF ANTINUCLEAR ANTIBODY (ANA) DETERMINATIONAnnals of the New York Academy of Sciences, 177
-
Robert Silber, Roberto Benitez, Warren Eveland, Joseph Akeroyd, Charles Dunne (1960)
The application of fluorescent antibody methods to the study of platelets.Blood, 16
-
Conns Conns, Kaplan Kaplan (1950)
Localisation of .antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibodyJ. exp. Med., 91
-
R. Mcmillan, Richard Smith, R. Longmire, R. Yelenosky, R. Reid, C. Craddock (1971)
Immunoglobulins associated with human platelets.Blood, 37 3
-
B. Pittman, G. Herbert, W. Cherry, G. Taylor (1967)
The quantitation of nonspecific staining as a guide for improvement of fluorescent antibody conjugates.Journal of immunology, 98 6
-
J. Veen, A. Kuivenhoven, T. Feltkamp (1971)
AN APPROACH TOWARD AUTOMATION OF ANTINUCLEAR ANTIBODY (ANA) DETERMINATIONAnnals of the New York Academy of Sciences, 177
-
M. Goldman (1967)
AN IMPROVED MICROFLUORIMETER FOR MEASURING BRIGHTNESS OF FLUORESCENT ANTIBODY REACTIONSJournal of Histochemistry and Cytochemistry, 15
-
(1971)
Studies on Received 1 I December 1972 Received in revised fortn 24 February 1973 the standardization of antinuclear antibtxJics ( ANA ) determination -
C. Engelfriet, A. Borne, M. Giessen, D. Beckers, J. Loghem (1968)
Autoimmune haemolytic anaemias. I. Serological studies with pure anti-immunoglobulin reagents.Clinical and experimental immunology, 3 7
-
H. Denk, M. Fischer, H. Pilgerstorfer, H. Schnack (1969)
Immunfluoreszenzmikrophotometrie zur quantitativen Erfassung antithrombozytärer AutoantikörperThrombosis and Haemostasis, 21
-
C. Mueller-Eckhardt, V. Sachs (1966)
Untersuchungen zum Nachweis antithrombozytärer Heteroantikörper durch ImmunfluoreszenzThrombosis and Haemostasis, 16
-
R. Nairn, F. Herzog, H. Ward, W. Boer (1969)
Microphotometry in immunofluorescence.Clinical and experimental immunology, 4 6
-
Mueller‐Eckhardt Mueller‐Eckhardt, Sachs Sachs (1966)
Untersuchungen zum Nachweis antithrombocytärer Heteroantikörper durch ImmunofluoreszenzThrombos. Diathes. haemorrh., 16
-
(1972)
Phiteht Atiloanlibodies; Methodological and Clinical Studies. Thesis, Amsterdam -
Corn Corn, Upshaw Upshaw (1962)
Evaluation of platelet antibodies in idiopathic thrombocytopenic purpuraArch. intern. Med., 109
-
G. Hébert, B. Pittman, W. Cherry (1967)
Factors affecting the degree of nonspecific staining given by fluorescein isothiocyanate labelled globulins.Journal of immunology, 98 6
-
Goldman Goldman (1967)
An improved microfluorimeter for measuring brightness of fluorescent antibody reactionsH. Histochem. Cytochem., 15
-
M. Corn, J. Upshaw (1962)
Evaluation of Platelet Antibodies in Idiopathic Thrombocytopenic PurpuraJAMA Internal Medicine, 109
-
T. The, T. Feltkamp (1970)
Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method.Immunology, 18 6
-
(1955)
Aspect dynamique des plaquettes sanguines 4 I'etat normal et pathologique -
Denk Denk, Fischer Fischer, Pilgerstorfer Pilgerstorfer, Schnack Schnack (1969)
Immunofluoreszenzmicrophotometrie zur quantitativen Erfassung anti‐thrombocytärer AutoantikörperThrombos. Diathes. haemorrh, 21
-
W. Irvine, M. Chan, W. Williamson (1969)
The application of microphotometry to the indirect immunofluorescent antibody technique using the human gastric parietal cell antigen-antibody system.Clinical and experimental immunology, 4 5
- Publisher
- Wiley
- Copyright
- Copyright © 1973 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0300-9475
- eISSN
- 1365-3083
- DOI
- 10.1111/j.1365-3083.1973.tb02032.x
- Publisher site
- See Article on Publisher Site
Abstract
The standardization is described of an immunofluorescent microphotometric method that offers a solution for the problems generally encountered in immunofluorescence procedures on platelets. Fluorescein isothiocyanate (FITC) conjugated .anti‐immunoglobulin serum and anti‐IgG serum were used. Sera, containing isoor hetero‐antibodies against platelets that had given positive reactions with one or more of the conventional serological techniques, were used as positive controls. The method described proved to have a good reproducibility. Significant differences in fluorescence were obtained using the above‐mentioned positive sera and normal control sera. It was found that platelets stored in sodium‐azide could be used as antigen. A number of variables that may influence the difference in fluorescence obtained with positive and normal sera were studied.
Journal
Scandinavian Journal of Immunology – Wiley
Published: May 1, 1973