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Eur J Clin Chem Clin Biochem 1996; 34:125-131 © 1996 by Walter de Gruyter · Berlin · New York Conditions for Single Strand Conformation Polymorphism (SSCP) Analysis with Broad Applicability: A Study on the Effects of Acrylamide, Buffer and Glycerol Concentrations in SSCP Analysis of Exons of the p53 Gene1) Winfried Teschauer{, Thomas Mussack2, Andreas Braun1, Helmut Waldner'2· and Edwin Fink[ Abteilung f r Klinische Chemie und Klinische Biochemie in der Chirurgischen Klinik und Poliklinik Chirurgische Klinik und Poliklinik, Klinikum Innenstadt Abteilung f r Klinische Chemie, Dr. v. Haunersches Kinderspital Ludwig-Maximilians-Universit t, M nchen, Germany Summary: We examined the influence of electrophoretic conditions on the detectability of small sequence alterations in DNA fragments by single strand conformation polymorphism (SSCP) analysis. Three acrylamide concentrations, 7.5, 14 and 20%, were selected, and all three gel types were prepared with four different Tris/borate/EDTA buffer concentrations. In addition, all these twelve gels were prepared both with and without 10% glycerol. All electrophoretic runs were performed at ambient temperature of 20-24 °C. The resulting 24 different electrophoretic conditions were used for the SSCP analysis of DNA fragments of exons 5, 7 and 8 of the human p53 gene; the results were evaluated primarily
Clinical Chemistry and Laboratory Medicine – de Gruyter
Published: Jan 1, 1996
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