Access the full text.
Sign up today, get DeepDyve free for 14 days.
DR Gallie, WJ Lucas, V Walbot (1989)
Visualizing mRNA expression in plant protoplasts: factors influencing efficient mRNA uptake and translationJ Bacteriol, 1
M‐H Chen, J Sheng, G Hind, A Handa, V Citovsky (2000)
Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell‐to‐cell movementJ Bacteriol, 19
B. Guralnick, G. Thomsen, V. Citovsky (1996)
Transport of DNA into the nuclei of xenopus oocytes by a modified VirE2 protein of Agrobacterium.The Plant cell, 8
T. Tzfira, Y. Rhee, M. Chen, T. Kunik, V. Citovsky (2000)
Nucleic acid transport in plant-microbe interactions: the molecules that walk through the walls.Annual review of microbiology, 54
J. Nam, K. Mysore, C. Zheng, M. Knue, A. Matthysse, S. Gelvin (1999)
Identification of T-DNA tagged Arabidopsis mutants that are resistant to transformation by AgrobacteriumMolecular and General Genetics MGG, 261
Note added in proof After submission of this manuscript, Tz®ra and Citovsky (2001) showed that nopaline-and octopine-speci®c VirE2 proteins exhibit similar features of nuclear import in living cells
M De Neve, S De Buck, A Jacobs, M Van Montagu, A Depicker (1997)
T‐DNA integration patterns in co‐transformed plant cells suggest that T‐DNA repeats originate from co‐integration of separate T‐DNAsTrends Biochem Sci, 11
SB Gelvin (1998)
Agrobacterium VirE2 proteins can form a complex with T strands in the plant cytoplasmAnnu Rev Plant Physiol Plant Mol Biol, 180
Maria Restrepo, D. Freed, James Carrington (1990)
Nuclear transport of plant potyviral proteins.The Plant cell, 2
C. Dingwall, R. Laskey (1991)
Nuclear targeting sequences--a consensus?Trends in biochemical sciences, 16 12
T. Tzfira, V. Citovsky (2000)
From host recognition to T-DNA integration: the function of bacterial and plant genes in the Agrobacterium-plant cell interaction.Molecular plant pathology, 1 4
V. Citovsky, Debra Warnick, Patricia Zambryski (1994)
Nuclear import of Agrobacterium VirD2 and VirE2 proteins in maize and tobacco.Proceedings of the National Academy of Sciences of the United States of America, 91
L. Pemberton, J. Rosenblum, G. Blobel (1999)
Nuclear Import of the TATA-binding Protein: Mediation by the Karyopherin Kap114p and a Possible Mechanism for Intranuclear TargetingThe Journal of Cell Biology, 145
T. Durfee, K. Becherer, P. Chen, S. Yeh, Y. Yang, A. Kilburn, W. Lee, S. Elledge (1993)
The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.Genes & development, 7 4
John Zupan, Patricia Zambryski, V. Citovsky (1997)
The Agrobacterium DNA Transfer ComplexCritical Reviews in Plant Sciences, 16
C Dingwall, RA Laskey (1991)
Nuclear targeting sequences—a consensus?FEBS Lett, 16
M. McPherson, B. Hames, G. Taylor (2016)
PCR 2 : a practical approach
CE Shurvinton, L Hodges, W Ream (1992)
A nuclear localization signal and the C‐terminal omega sequence in the Agrobacterium tumefaciens VirD2 endonuclease are important for tumor formationMol Gen Genet, 89
A Herrera‐Estrella, M Van Montagu, K Wang (1990)
A bacterial peptide acting as a plant nuclear targeting signal: the amino‐terminal portion of Agrobacterium virD2 protein directs a β‐galactosidase fusion protein into tobacco nucleiMol Cell Biol, 87
S. Altschul, W. Gish, W. Miller, E. Myers, D. Lipman (1990)
Basic local alignment search tool.Journal of molecular biology, 215 3
P. Sen, G. Pazour, D. Anderson, A. Das (1989)
Cooperative binding of Agrobacterium tumefaciens VirE2 protein to single-stranded DNAJournal of Bacteriology, 171
John Zupan, V. Citovsky, Patricia Zambryski (1996)
Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA in plant cells.Proceedings of the National Academy of Sciences of the United States of America, 93 6
T. Murashige, F. Skoog (1962)
A revised medium for rapid growth and bio assays with tobacco tissue culturesPhysiologia Plantarum, 15
J. Carrington, D. Freed, Andrew Leinicke (1991)
Bipartite signal sequence mediates nuclear translocation of the plant potyviral NIa protein.The Plant cell, 3 9
B. Tinland, B. Hohn (1995)
Recombination between prokaryotic and eukaryotic DNA: integration of Agrobacterium tumefaciens T-DNA into the plant genome.Genetic engineering, 17
Min‐Huei Chen, J. Sheng, G. Hind, A. Handa, V. Citovsky (2000)
Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell‐to‐cell movementThe EMBO Journal, 19
C Gietl, Z Koukolikova‐Nicola, B Hohn (1987)
Mobilization of T‐DNA from Agrobacterium to plant cells involves a protein that binds single‐stranded DNAPlant Cell, 84
John Zupan, T. Muth, Olga Draper, P. Zambryski (2000)
The transfer of DNA from agrobacterium tumefaciens into plants: a feast of fundamental insights.The Plant journal : for cell and molecular biology, 23 1
V Citovsky, B Guralnick, MN Simon, JS Wall (1997)
The molecular structure of Agrobacterium VirE2–single stranded DNA complexes involved in nuclear importProc Natl Acad Sci USA, 271
K. Schiene, A. Pühler, K. Niehaus (2000)
Transgenic tobacco plants that express an antisense construct derived from a Medicago sativa cDNA encoding a Rac-related small GTP-binding protein fail to develop necrotic lesions upon elicitor infiltrationMolecular and General Genetics MGG, 263
M Thangavelu, D Belostotsky, MW Bevan, RB Flavell, HJ Rogers, DM Lonsdale (1993)
Partial characterization of the Nicotiana tabacum actin gene family: evidence for pollen‐specific expression of one of the gene family membersAnnu Rev Microbiol, 240
T Oku, JG Tjuvajev, T Miyagawa, T Sasajima, A Joshi, R Joshi, R Finn, KP Claffey, RG Blasberg (1998)
Tumor growth modulation by sense and antisense vascular endothelial growth factor gene expression: effects on angiogenesis, vascular permeability, blood volume, blood flow, fluorodeoxyglucose uptake and proliferation of human melanoma intracerebral xenograftsPlant Cell, 58
V. Citovsky, M. Wong, P. Zambryski (1989)
Cooperative interaction of Agrobacterium VirE2 protein with single-stranded DNA: implications for the T-DNA transfer process.Proceedings of the National Academy of Sciences of the United States of America, 86 4
(1987)
Current Protocols in Molecular Biology
L. Rossi, B. Hohn, B. Tinland (1993)
The VirD2 protein of Agrobacterium tumefaciens carries nuclear localization signals important for transfer of T-DNA to plant.Molecular & general genetics : MGG, 239 3
Z Koukolikova‐Nicola, D Raineri, K Stephens, C Ramos, B Tinland, EW Nester, B Hohn (1993)
Genetic analysis of the virD operon of Agrobacterium tumefaciens: a search for functions involved in transport of T‐DNA into the plant cell nucleus and in T‐DNA integrationPhysiol Plant, 175
KM Kironmai, K Muniyappa, DB Friedman, NM Hollingsworth, B Byers (1998)
DNA‐binding activities of Hop1 protein, a synaptonemal complex component from Saccharomyces cerevisiaeJ Mol Biol, 18
M. Stam, R. Bruin, R. Blokland, R. Hoorn, J. Mol, J. Kooter (2000)
Distinct features of post-transcriptional gene silencing by antisense transgenes in single copy and inverted T-DNA repeat loci.The Plant journal : for cell and molecular biology, 21 1
E. Howard, John Zupan, V. Citovsky, P. Zambryski (1992)
The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: Implications for nuclear uptake of DNA in plant cellsCell, 68
T. Lohman, Leslie Overman, S. Datta (1986)
Salt-dependent changes in the DNA binding co-operativity of Escherichia coli single strand binding protein.Journal of molecular biology, 187 4
M. Rout, S. Wente (1994)
Pores for thought: nuclear pore complex proteins.Trends in cell biology, 4 10
S. Stachel, P. Zambryski (1989)
Generic trans-kingdom sex?Nature, 340
SB Gelvin (2000)
Agrobacterium and plant genes involved in T‐DNA transfer and integrationProc Natl Acad Sci USA, 51
T Murashige, F Skoog (1962)
A revised medium for rapid growth and bio assays with tobacco tissue culturesCancer Res, 15
Y. Dorokhov, K. Mäkinen, O. Frolova, Andres Merits, J. Saarinen, Nisse Kalkkinen, J. Atabekov, Mart Saarma (1999)
A novel function for a ubiquitous plant enzyme pectin methylesterase: the host‐cell receptor for the tobacco mosaic virus movement proteinFEBS Letters, 461
Robert Kraft, J. Tardiff, Kenneth Krauter, L. Leinwand (1988)
Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase.BioTechniques, 6 6
S. Stachel, P. Zambryski (1989)
Bacteria-yeast conjugation. Generic trans-kingdom sex?Nature, 340 6230
B Tinland, B Hohn (1995)
Recombination between prokaryotic and eukaryotic DNA: integration of Agrobacterium tumefaciens T‐DNA into the plant genomePlant Cell, 17
V. Citovsky, D. Knorr, G. Schuster, P. Zambryski (1990)
The P30 movement protein of tobacco mosaic virus is a single-strand nucleic acid binding proteinCell, 60
H Vaucheret, C Beclin, T Elmayan, F Feuerbach, C Godon, JB Morel, P Mourrain, JC Palauqui, S Vernhettes (1998)
Transgene‐induced gene silencing in plantsCrit Rev Plant Sci, 16
K. Kironmai, K. Muniyappa, K. Muniyappa, D. Friedman, N. Hollingsworth, B. Byers (1998)
DNA-Binding Activities of Hop1 Protein, a Synaptonemal Complex Component from Saccharomyces cerevisiaeMolecular and Cellular Biology, 18
MP Rout, SR Wente (1994)
Pores for thought: nuclear pore complex proteinsJ Bacteriol, 4
P. Bartel, Cheng-Ting Chien, Rolf Sternglanz, Stanley Fields (1993)
Elimination of false positives that arise in using the two-hybrid system.BioTechniques, 14 6
LF Pemberton, JS Rosenblum, G Blobel (1999)
Nuclear import of the TATA‐binding protein: mediation by the karyopherin Kap114p and a possible mechanism for intranuclear targetingMol Gen Genet, 145
V. Citovsky, B. Guralnick, M. Simon, J. Wall (1997)
The molecular structure of agrobacterium VirE2-single stranded DNA complexes involved in nuclear import.Journal of molecular biology, 271 5
T Tzfira, Y Rhee, M‐H Chen, V Citovsky (2000)
Nucleic acid transport in plant–microbe interactions: the molecules that walk through the wallsAnnu Rev Plant Physiol Plant Mol Biol, 54
JC Carrington, DD Freed, AJ Leinicke (1991)
Bipartite signal sequence mediates nuclear translocation of the plant potyviral NIa proteinEMBO J, 3
M. Neve, S. Buck, A. Jacobs, M. Montagu, A. Depicker (1997)
T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs.The Plant journal : for cell and molecular biology, 11 1
Z. Koukolíková-Nicola, D. Raineri, K. Stephens, C. Ramos, B. Tinland, E. Nester, B. Hohn (1993)
Genetic analysis of the virD operon of Agrobacterium tumefaciens: a search for functions involved in transport of T-DNA into the plant cell nucleus and in T-DNA integrationJournal of Bacteriology, 175
R. Horsch, J. Fry, N. Hoffman, D. Eichholtz, S. Rogers, R. Fraley (1985)
A simple and general method for transferring genes into plants.Science, 227 4691
N. Saitou, M. Nei (1987)
The neighbor-joining method: a new method for reconstructing phylogenetic trees.Molecular biology and evolution, 4 4
J. Sheng, V. Citovsky (1996)
Agrobacterium-plant cell DNA transport: have virulence proteins, will travel.The Plant cell, 8
C. Shurvinton, L. Hodges, W. Ream (1992)
A nuclear localization signal and the C-terminal omega sequence in the Agrobacterium tumefaciens VirD2 endonuclease are important for tumor formation.Proceedings of the National Academy of Sciences of the United States of America, 89 24
A Ziemienowicz, D Gorlich, E Lanka, B Hohn, L Rossi (1999)
Import of DNA into mammalian nuclei by proteins originating from a plant pathogenic bacteriumPlant J, 96
M. Ni, J. Tepperman, P. Quail (1998)
PIF3, a Phytochrome-Interacting Factor Necessary for Normal Photoinduced Signal Transduction, Is a Novel Basic Helix-Loop-Helix ProteinCell, 95
VirE2-interacting protein in Agrobacterium tumorigenicity
N Saitou, M Nei (1987)
The neighbor joining method: A new method for reconstructing phylogenetic treesPlant Cell, 4
K Schiene, A Puhler, K Niehaus (2000)
Transgenic tobacco plants that express an antisense construct derived from a Medicago sativa cDNA encoding a Rac‐related small GTP‐binding protein fail to develop necrotic lesions upon elicitor infiltrationProc Natl Acad Sci USA, 263
J Sheng, V Citovsky (1996)
Agrobacterium–plant cell interaction: have virulence proteins—will travelPlant J, 8
S. Fields, O. Song (1989)
A novel genetic system to detect proteinprotein interactionsNature, 340
P. Christie, J. Ward, S. Winans, E. Nester (1988)
The Agrobacterium tumefaciens virE2 gene product is a single-stranded-DNA-binding protein that associates with T-DNAJournal of Bacteriology, 170
H. Vaucheret, C. Beclin, T. Elmayan, F. Feuerbach, C. Godon, J. Morel, P. Mourrain, J. Palauqui, S. Vernhettes (1998)
Transgene-induced gene silencing in plants.The Plant journal : for cell and molecular biology, 16 6
V. Citovsky, Guido VOS, P. Zambryski (1988)
Single-Stranded DNA Binding Protein Encoded by the virE Locus of Agrobacterium tumefaciensScience, 240
S. Hollenberg, R. Sternglanz, P. Cheng, H. Weintraub (1995)
Identification of a new family of tissue-specific basic helix-loop-helix proteins with a two-hybrid systemMolecular and Cellular Biology, 15
T Tzfira, V Citovsky (2000)
From host recognition to T‐DNA integration: the function of bacterial and plant genes in the Agrobacterium–plant cell interactionPlant J, 1
A. Beijersbergen, P. Hooykaas (1994)
The Virulence System of Agrobacterium TumefaciensAnnual Review of Phytopathology, 32
S. Gelvin (1998)
Agrobacterium VirE2 Proteins Can Form a Complex with T Strands in the Plant CytoplasmJournal of Bacteriology, 180
SM Hollenberg, R Sternglanz, PF Cheng, H Weintraub (1995)
Identification of a new family of tissue‐specific basic helix–loop–helix proteins with a two‐hybrid systemAnnu Rev Phytopathol, 15
A Das (1988)
Agrobacterium tumefaciens virE operon encodes a single‐stranded DNA‐binding proteinPlant J, 85
F. Sherman, G. Fink, J. Hicks (1979)
Methods in yeast genetics
P. Zambryski (1992)
Chronicles from the Agrobacterium-plant cell DNA transfer story, 43
Genes Dev
AR van der Krol, N‐H Chua (1991)
The basic domain of plant B‐ZIP proteins facilitates import of a reporter protein into plant nucleiProc Natl Acad Sci USA, 3
V Citovsky, D Warnick, P Zambryski (1994)
Nuclear import of Agrobacterium VirD2 and VirE2 proteins in maize and tobaccoJ Mol Biol, 91
B Guralnick, G Thomsen, V Citovsky (1996)
Transport of DNA into the nuclei of Xenopus oocytes by a modified VirE2 protein of AgrobacteriumProc Natl Acad Sci USA, 8
MA Restrepo, DD Freed, JC Carrington (1990)
Nuclear transport of plant potyviral proteinsTrends Cell Biol, 2
V. Citovsky, John Zupan, D. Warnick, P. Zambryski (1992)
Nuclear localization of Agrobacterium VirE2 protein in plant cells.Science, 256 5065
Y Rhee, F Gurel, Y Gafni, C Dingwall, V Citovsky (2000)
A genetic system for detection of protein nuclear import and exportMol Biol Evol, 18
Takamitsu Oku, J. Tjuvajev, T. Miyagawa, T. Sasajima, A. Joshi, R. Joshi, R. Finn, K. Claffey, R. Blasberg (1998)
Tumor growth modulation by sense and antisense vascular endothelial growth factor gene expression: effects on angiogenesis, vascular permeability, blood volume, blood flow, fluorodeoxyglucose uptake, and proliferation of human melanoma intracerebral xenografts.Cancer research, 58 18
A. Herrera-Estrella, M. Montagu, K. Wang (1990)
A bacterial peptide acting as a plant nuclear targeting signal: the amino-terminal portion of Agrobacterium VirD2 protein directs a beta-galactosidase fusion protein into tobacco nuclei.Proceedings of the National Academy of Sciences of the United States of America, 87 24
Anath Das (1988)
Agrobacterium tumefaciens virE operon encodes a single-stranded DNA-binding protein.Proceedings of the National Academy of Sciences of the United States of America, 85 9
Nurit Ballas, V. Citovsky (1997)
Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein.Proceedings of the National Academy of Sciences of the United States of America, 94 20
Christine Gietl, Z. Koukolíková-Nicola, Barbara Hohn (1987)
Mobilization of T-DNA from Agrobacterium to plant cells involves a protein that binds single-stranded DNA.Proceedings of the National Academy of Sciences of the United States of America, 84 24
D. Gallie, William Lucas, Virginia Walbot (1989)
Visualizing mRNA expression in plant protoplasts: factors influencing efficient mRNA uptake and translation.The Plant cell, 1
Y. Rhee, F. Gurel, Y. Gafni, C. Dingwall, V. Citovsky (2000)
A genetic system for detection of protein nuclear import and exportNature Biotechnology, 18
Hangil Park, R. Sternglanz (1998)
Two separate conserved domains of eukaryotic DNA topoisomerase I bind to each other and reconstitute enzymatic activityChromosoma, 107
S. Gelvin (2000)
AGROBACTERIUM AND PLANT GENES INVOLVED IN T-DNA TRANSFER AND INTEGRATION.Annual review of plant physiology and plant molecular biology, 51
SF Altschul, W Gish, W Miller, EW Myers, DJ Lipman (1990)
Basic local alignment toolJ Mol Biol, 215
Alexander Krol, Nam-Hai, Chua (1991)
The basic domain of plant B-ZIP proteins facilitates import of a reporter protein into plant nuclei.The Plant cell, 3
M Stam, R de Bruin, R van Blokland, RA van der Hoorn, JN Mol, JM Kooter (2000)
Distinct features of post‐transcriptional gene silencing by antisense transgenes in single copy and inverted T‐DNA repeat lociMol Plant Pathol, 21
BJ Janssen, RC Gardner (1990)
Localized transient expression of GUS in leaf discs following cocultivation with AgrobacteriumMol Cell Biol, 14
J Zupan, V Citovsky, P Zambryski (1996)
Agrobacterium VirE2 protein mediates nuclear uptake of ssDNA in plant cells, 93
A. Ziemienowicz, Dirk Görlich, Erich Lanka, Barbara Hohn, Luca Rossi (1999)
Import of DNA into mammalian nuclei by proteins originating from a plant pathogenic bacterium.Proceedings of the National Academy of Sciences of the United States of America, 96 7
YL Dorokhov, K Makinen, OY Frolova, A Merits, J Saarinen, N Kalkkinen, JG Atabekov, M Saarma (1999)
A novel function for a ubiquitous plant enzyme pectin methylesterase: the host‐cell receptor for the tobacco mosaic virus movement proteinGenes Dev, 461
(1995)
Quanti®cation of DNA and RNA by PCR
(2000)
Received December
T‐DNA nuclear import is a central event in genetic transformation of plant cells by Agrobacterium. This event is thought to be mediated by two bacterial proteins, VirD2 and VirE2, which are associated with the transported T‐DNA molecule. While VirD2 is imported into the nuclei of plant, animal and yeast cells, nuclear uptake of VirE2 occurs most efficiently in plant cells. To understand better the mechanism of VirE2 action, a cellular interactor of VirE2 was identified and its encoding gene cloned from Arabidopsis. The identified plant protein, designated VIP1, specifically bound VirE2 and allowed its nuclear import in non‐plant systems. In plants, VIP1 was required for VirE2 nuclear import and Agrobacterium tumorigenicity, participating in early stages of T‐DNA expression.
The EMBO Journal – Wiley
Published: Feb 2, 2001
Keywords: ; ; ; ;
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.