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- Publisher
- Wiley
- Copyright
- Copyright © 1993 Wiley Subscription Services, Inc., A Wiley Company
- ISSN
- 0950-382X
- eISSN
- 1365-2958
- DOI
- 10.1111/j.1365-2958.1993.tb01131.x
- Publisher site
- See Article on Publisher Site
Abstract
Summary Genetic studies of Mycobacterium tuberculosis and other mycobacterial pathogens have suffered from the lack of a sophisticated genetic system. To address this issue we have developed a viral system through a detailed characterization of mycobacteriophage L5, a temperate phage that infects both fast‐ and slow‐growing mycobacteria. We describe here the complete DNA sequence of the L5 genome and initial characterization of L5 virion structure and gene expression. In addition to providing a genetic‘toolbox’for the mycobacteria we find that L5 offers a new paradigm for dsDNA phages, being phenotypically temperate but employing genetic strategies for phage growth usually associated with lytic bacteriophages.
Journal
Molecular Microbiology – Wiley
Published: Feb 1, 1993