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THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY

THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY Key to Abbreviations Ch, chromatin G, Golgi apparatus Gly, glycogen L, acinar lumen Mi, microbody Mt, mitochondria FIGURE ] N, nucleus Np, nuclear pore Nu, nucleolus Rer, granular endoplasmie reticulum Ser, agranular endoplasmic reticulum Z, zymogen granule Portion of an acinus of pancreas of rat. Note the virtual absence of stain contamination. Phosphate-buffered osmium tetroxide fixation (9), Epon embedding. Stained with lead citrate 5 minutes. X 9,000. FIGURE Portion of cytoplasm of a parcnchymal cell of liver of rat. For the most part in this micrograph glycogen appears as finely granular deposits of less electron opacity than ribosomes in contiguous areas. Glycogen dcposlts staining more intensely are indicated by arrows. In thicker sections prepared for electron microscopy, glycogen deposits stain more intensely than ribosomes. Fixation and embedding as in Fig. 1. Stained with a 1 : 5 dilution of lead citrate 5 minutes. )< 25,000. 2~ placing 1.33 gm Pb(NO3)2, t 1.76 g m Na3 (C6H507) • 2H20 t and 30 ml distilled water in a 50 ml volumetric flask. The resultant suspension is shaken vigorously for 1 minute and allowed to stand with intermittent shaking in order to insure complete conversion of lead nitrate to lead citrate. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY

The Journal of Cell Biology , Volume 17 (1): 208 – Apr 1, 1963

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Publisher
Rockefeller University Press
Copyright
Copyright © 1963 by The Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.17.1.208
Publisher site
See Article on Publisher Site

Abstract

Key to Abbreviations Ch, chromatin G, Golgi apparatus Gly, glycogen L, acinar lumen Mi, microbody Mt, mitochondria FIGURE ] N, nucleus Np, nuclear pore Nu, nucleolus Rer, granular endoplasmie reticulum Ser, agranular endoplasmic reticulum Z, zymogen granule Portion of an acinus of pancreas of rat. Note the virtual absence of stain contamination. Phosphate-buffered osmium tetroxide fixation (9), Epon embedding. Stained with lead citrate 5 minutes. X 9,000. FIGURE Portion of cytoplasm of a parcnchymal cell of liver of rat. For the most part in this micrograph glycogen appears as finely granular deposits of less electron opacity than ribosomes in contiguous areas. Glycogen dcposlts staining more intensely are indicated by arrows. In thicker sections prepared for electron microscopy, glycogen deposits stain more intensely than ribosomes. Fixation and embedding as in Fig. 1. Stained with a 1 : 5 dilution of lead citrate 5 minutes. )< 25,000. 2~ placing 1.33 gm Pb(NO3)2, t 1.76 g m Na3 (C6H507) • 2H20 t and 30 ml distilled water in a 50 ml volumetric flask. The resultant suspension is shaken vigorously for 1 minute and allowed to stand with intermittent shaking in order to insure complete conversion of lead nitrate to lead citrate.

Journal

The Journal of Cell BiologyRockefeller University Press

Published: Apr 1, 1963

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