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Key to Abbreviations Ch, chromatin G, Golgi apparatus Gly, glycogen L, acinar lumen Mi, microbody Mt, mitochondria FIGURE ] N, nucleus Np, nuclear pore Nu, nucleolus Rer, granular endoplasmie reticulum Ser, agranular endoplasmic reticulum Z, zymogen granule Portion of an acinus of pancreas of rat. Note the virtual absence of stain contamination. Phosphate-buffered osmium tetroxide fixation (9), Epon embedding. Stained with lead citrate 5 minutes. X 9,000. FIGURE Portion of cytoplasm of a parcnchymal cell of liver of rat. For the most part in this micrograph glycogen appears as finely granular deposits of less electron opacity than ribosomes in contiguous areas. Glycogen dcposlts staining more intensely are indicated by arrows. In thicker sections prepared for electron microscopy, glycogen deposits stain more intensely than ribosomes. Fixation and embedding as in Fig. 1. Stained with a 1 : 5 dilution of lead citrate 5 minutes. )< 25,000. 2~ placing 1.33 gm Pb(NO3)2, t 1.76 g m Na3 (C6H507) ⢠2H20 t and 30 ml distilled water in a 50 ml volumetric flask. The resultant suspension is shaken vigorously for 1 minute and allowed to stand with intermittent shaking in order to insure complete conversion of lead nitrate to lead citrate.
The Journal of Cell Biology – Rockefeller University Press
Published: Apr 1, 1963
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