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J. Bergeron, B. Hébert, P. Tijssen (1996)
Genome organization of the Kresse strain of porcine parvovirus: identification of the allotropic determinant and comparison with those of NADL-2 and field isolatesJournal of Virology, 70
N. Burgess-Brown, Sujata Sharma, F. Sobott, Christoph Loenarz, U. Oppermann, O. Gileadi (2008)
Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study.Protein expression and purification, 59 1
P. Michel, Anna Mäkelä, Eila Korhonen, J. Toivola, L. Hedman, M. Söderlund-Venermo, K. Hedman, C. Oker‐Blom (2008)
Purification and analysis of polyhistidine-tagged human parvovirus B19 VP1 and VP2 expressed in insect cells.Journal of virological methods, 152 1-2
J. Turiso, E. Cortés, A. Ranz, J. Garcia, A. Sanz, C. Vela, J. Casal (1991)
Fine mapping of canine parvovirus B cell epitopes.The Journal of general virology, 72 ( Pt 10)
J. Casal (2004)
Parvovirus diagnostics and vaccine production in insect cellsCytotechnology, 20
S. Krakowka, J. Ellis, B. Meehan, S. Kennedy, F. Mcneilly, G. Allan (2000)
Viral Wasting Syndrome of Swine: Experimental Reproduction of Postweaning Multisystemic Wasting Syndrome in Gnotobiotic Swine by Coinfection with Porcine Circovirus 2 and Porcine ParvovirusVeterinary Pathology, 37
Qunxing Pan, K. He, Kehe Huang (2008)
Development of recombinant porcine parvovirus-like particles as an antigen carrier formed by the hybrid VP2 protein carrying immunoreactive epitope of porcine circovirus type 2.Vaccine, 26 17
C. Martinez, K. Dalsgaard, J. Turiso, Elena Cortés, C. Vela, J. Casal (1992)
Production of porcine parvovirus empty capsids with high immunogenic activity.Vaccine, 10 10
Y. Hu, W. Bentley, G. Edwards, V. Vakharia (1999)
Chimeric infectious bursal disease virus-like particles expressed in insect cells and purified by immobilized metal affinity chromatography.Biotechnology and bioengineering, 63 6
A. Mayr, P. Bachmann, G. Siegl, H. Mahnel, B. Sheffy (2005)
Characterization of a small Porcine DNA virusArchiv für die gesamte Virusforschung, 25
(1975)
Gesamte Virusforsch
A. Simpson, B. Hébert, G. Sullivan, C. Parrish, Z. Zádori, P. Tijssen, M. Rossmann (2002)
The structure of porcine parvovirus: comparison with related viruses.Journal of molecular biology, 315 5
J. Segalés, G. Allan, M. Domingo (2005)
Porcine circovirus diseasesAnimal Health Research Reviews, 6
Mengeling Wl, Cutlip Rc (1975)
Pathogenesis of in utero infection: experimental infection of five-week-old porcine fetuses with porcine parvovirus.American Journal of Veterinary Research, 36
C. Choi, T. Molitor, H. Joo, R. Gunther (1987)
Pathogenicity of a skin isolate of porcine parvovirus in swine fetuses.Veterinary microbiology, 15 1-2
Rodrigo Soares, E. Durigon, J. Bersano, L. Richtzenhain (1999)
Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1.Journal of virological methods, 78 1-2
R. Lo‐Man, P. Rueda, C. Sedlik, E. Dériaud, I. Casal, C. Leclerc (1998)
A recombinant virus‐like particle system derived from parvovirus as an efficient antigen carrier to elicit a polarized Th1 immune response without adjuvantEuropean Journal of Immunology, 28
J. Kresse, W. Taylor, W. Stewart, K. Eernisse (1985)
Parvovirus infection in pigs with necrotic and vesicle-like lesions.Veterinary microbiology, 10 6
J. Park, Bong Choi, L. Vijayachandran, Vasudevan Ayyappan, C. Chong, Ki-Sung Lee, Sei-Chang Kim, C. Choi (2007)
Immunodetection of Canine Parvovirus (CPV) in clinical samples by polyclonal antisera against CPV-VP2 protein expressed in Esherichia coli as an antigen.Journal of virological methods, 146 1-2
J. Pal, Dhanashri Godbole, K. Sharma (2004)
Staining of proteins on SDS polyacrylamide gels and on nitrocellulose membranes by Alta, a colour used as a cosmetic.Journal of biochemical and biophysical methods, 61 3
G. Allan, S. Kennedy, F. Mcneilly, J. Foster, J. Ellis, S. Krakowka, B. Meehan, B. Adair (1999)
Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus.Journal of comparative pathology, 121 1
J. Casal (1999)
Use of parvovirus‐like particles for vaccination and induction of multiple immune responsesBiotechnology and Applied Biochemistry, 29
Porcine parvovirus (PPV) is a widespread infectious virus that causes serious reproductive diseases of swine and death of piglets. The gene coding for the capsid protein VP2 of PPV was amplified and inserted into the plasmid pET-32a (+), which was then used to transform Escherichia coli Rosetta, the capsid protein of PPV was fused to a polyhistidine tag, and the position of the affinity tag is in N-terminus. VP2 was expressed using different expression host bacteria, including E. coli BL21, and Rosetta, and different plasmid vectors, including pET-30a (+), pET-32a (+), and pGEX-6p-1. After selection, only the fusion protein inserted into pET-32a (+) was expressed well in E. coli Rosetta. The recombinant bacterium produced high quantities of the fusion protein VP2, about 8% in total. The expressed VP2 was antigenically similar to the native capsid protein according to a Western blot assay performed with polyclonal antibodies obtained from pigs vaccinated with PPV. A simple, easily commercialized procedure was used to purify this protein. This study provides a foundation for the application of VP2 protein in the clinical diagnosis of PPV and in the vaccination against PPV.
Virus Genes – Springer Journals
Published: Jun 20, 2009
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