Access the full text.
Sign up today, get DeepDyve free for 14 days.
J. Kooter, M. Matzke, P. Meyer (1999)
Listening to the silent genes: transgene silencing, gene regulation and pathogen control.Trends in plant science, 4 9
Bradley Luff, Laura Pawlowski, J. Bender (1999)
An inverted repeat triggers cytosine methylation of identical sequences in Arabidopsis.Molecular cell, 3 4
L. Jones, A. Hamilton, O. Voinnet, Carole Thomas, A. Maule, D. Baulcombe (1999)
RNA–DNA Interactions and DNA Methylation in Post-Transcriptional Gene SilencingPlant Cell, 11
J. Paszkowski (2012)
Homologous Recombination and Gene Silencing in Plants
J. Bosher, M. Labouesse (2000)
RNA interference: genetic wand and genetic watchdogNature Cell Biology, 2
A. Fire, Siqun Xu, M. Montgomery, S. Kostas, Samuel Driver, C. Mello (1998)
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegansNature, 391
P. Mourrain, C. Beclin, T. Elmayan, F. Feuerbach, C. Godon, J. Morel, David Jouette, Anne-Marie Lacombe, Snezana Nikic, N. Picault, Karine Rémoué, Mathieu Sanial, T. Vo, H. Vaucheret (2000)
Arabidopsis SGS2 and SGS3 Genes Are Required for Posttranscriptional Gene Silencing and Natural Virus ResistanceCell, 101
D. Weigel, J. Ahn, M. Blázquez, J. Borevitz, Sioux Christensen, C. Fankhauser, C. Ferrándiz, I. Kardailsky, I. Kardailsky, Elizabeth Malancharuvil, M. Neff, M. Neff, Jasmine Nguyen, Shusei Sato, Zhi-Yong Wang, Yiji Xia, R. Dixon, M. Harrison, C. Lamb, C. Lamb, M. Yanofsky, J. Chory, J. Chory (2000)
Activation tagging in Arabidopsis.Plant physiology, 122 4
H. Tabara, Madathia Sarkissian, W. Kelly, J. Fleenor, A. Grishok, L. Timmons, A. Fire, C. Mello (1999)
The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegansCell, 99
J. Odell, S. Russell (1994)
Use of Site-Specific Recombination Systems in Plants
T. Dalmay, A. Hamilton, S. Rudd, S. Angell, D. Baulcombe (2000)
An RNA-Dependent RNA Polymerase Gene in Arabidopsis Is Required for Posttranscriptional Gene Silencing Mediated by a Transgene but Not by a VirusCell, 101
C. Cogoni, G. Macino (1999)
Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase.Science, 286 5448
C. Cogoni, G. Macino (1999)
Homology-dependent gene silencing in plants and fungi: a number of variations on the same theme.Current opinion in microbiology, 2 6
M. Wassenegger, S. Heimes, Leonhard Riedel, H. Sänger (1994)
RNA-directed de novo methylation of genomic sequences in plantsCell, 76
A Smardon, JM Spoerke, SC Stacey, ME Klein, N Mackin, EM Maine (2000)
EGO‐1 is related to RNA‐directed RNA polymerase and functions in germ‐line development and RNA interference in C.elegansPlant Cell, 10
Harry Smith (1999)
Interfering with Viral Infection: Plants Do It TooPlant Cell, 11
P. Waterhouse, M. Graham, Ming-Bo Wang (1998)
Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA.Proceedings of the National Academy of Sciences of the United States of America, 95 23
P. Sharp, P. Zamore (2000)
RNA InterferenceScience, 287
A. Smardon, J. Spoerke, Steven Stacey§, Marcia Klein¶, Nancy Mackin, E. Maine (2000)
EGO-1 is related to RNA-directed RNA polymerase and functions in germ-line development and RNA interference in C. elegansCurrent Biology, 10
R. Ketting, Thomas Haverkamp, H. Luenen, R. Plasterk (1999)
mut-7 of C. elegans, Required for Transposon Silencing and RNA Interference, Is a Homolog of Werner Syndrome Helicase and RNaseDCell, 99
S. Clough, A. Bent (1998)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 16 6
Madhur Kumar, G. Carmichael (1998)
Antisense RNA: Function and Fate of Duplex RNA in Cells of Higher EukaryotesMicrobiology and Molecular Biology Reviews, 62
EJ Finnegan, KA Kovac (2000)
Plant DNA methyltransferasesPlant Mol Biol, 43
P. Zamore, T. Tuschl, P. Sharp, D. Bartel (2000)
RNAi Double-Stranded RNA Directs the ATP-Dependent Cleavage of mRNA at 21 to 23 Nucleotide IntervalsCell, 101
M. Mette, J. Winden, M. Matzke, A. Matzke (1999)
Production of aberrant promoter transcripts contributes to methylation and silencing of unlinked homologous promoters in transThe EMBO Journal, 18
B. Symons (1989)
Pathogenesis by antisenseNature, 338
MA Matzke, F Neuhuber, AJM Matzke (1993)
A variety of epistatic interactions can occur between partially homologous transgene loci brought together by sexual crossingMol Gen Genet, 236
L. Lebruska, L. Maher (1999)
Selection and Characterization of an RNA Decoy for Transcription Factor NF-κB†Biochemistry, 38
C. Chuang, E. Meyerowitz (2000)
Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America, 97 9
H. Vaucheret (1993)
Identification of a general silencer for 19S and 35S promoters in a transgenic tobacco plant : 90 bp of homology in the promoter sequence are sufficient for trans-inactivation, 316
B. Bass (2000)
Double-Stranded RNA as a Template for Gene SilencingCell, 101
G. Faugeron (2000)
Diversity of homology-dependent gene silencing strategies in fungi.Current opinion in microbiology, 3 2
P Waterhouse, MW Graham, M‐B Wang (1998)
Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNAPlant Physiol, 95
M. Matzke, M. Primig, J. Trnovsky, A. Matzke (1989)
Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plantsThe EMBO Journal, 8
A. Jones, Carole Thomas, A. Maule (1998)
De novo methylation and co‐suppression induced by a cytoplasmically replicating plant RNA virusThe EMBO Journal, 17
MA Matzke, MF Mette, AJM Matzke (2000)
Transgene silencing by the host genome defense: implications for the evolution of epigenetic control mechanisms in plants and vertebratesPlant Mol Biol, 43
C. Cogoni, G. Macino (1999)
Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymeraseNature, 399
M Wassenegger (2000)
RNA‐directed DNA methylationCell, 43
J. Jakowitsch, I. Papp, E. Moscone, J. Winden, M. Matzke, A. Matzke (1999)
Molecular and cytogenetic characterization of a transgene locus that induces silencing and methylation of homologous promoters in trans.The Plant journal : for cell and molecular biology, 17 2
Andrew Hamilton, David Baulcombe (1999)
A species of small antisense RNA in posttranscriptional gene silencing in plants.Science, 286 5441
T. Pélissier, M. Wassenegger (2000)
A DNA target of 30 bp is sufficient for RNA-directed DNA methylation.RNA, 6 1
Double‐stranded RNA induces a post‐transcriptional gene silencing process, termed RNAi, in diverse organisms. It is shown here that transcriptional gene silencing accompanied by de novo methylation of a target promoter in plants can be triggered by a double‐stranded RNA containing promoter sequences. Similar to the double‐stranded RNA involved in RNAi, this promoter double‐stranded RNA, which is synthesized in the nucleus, is partially cleaved into small RNAs ∼23 nucleotides in length. Both transcriptional and post‐transcriptional gene silencing can thus be initiated by double‐stranded RNAs that enter the same degradation pathway. The results also implicate double‐stranded RNA in directing DNA methylation. Different constructs designed to produce double‐stranded promoter RNA in various ways were evaluated for their ability to induce gene silencing in tobacco and Arabidopsis. RNA hairpins transcribed from inverted DNA repeats were the most effective trans‐acting silencing signals. This strategy could be useful for transcriptionally downregulating genes in a variety of plants.
The EMBO Journal – Wiley
Published: Feb 2, 2000
Keywords: ; ; ; ;
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.