Strain Stimulations with Different Intensities on Fibroblast Viability and Protein Expression

Strain Stimulations with Different Intensities on Fibroblast Viability and Protein Expression AbstractBackgroundMechanical stimulation via acupuncture and tuina massage triggers various cell responses. This study aims to understand these cellular bio-physical mechanisms by investigating the effect of different stimulation intensities on cell viability and protein expression.MethodologyConnective tissue fibroblasts were cultured in vitro. Three varying intensities of mechanical strain stimulation were applied to the cells, either once or three times and compared with non-stimulated controls. Changes in fibroblast viability and fibroblast protein expression were observed.ResultsStrain stimulation intensity significantly increased fibroblast cell survival rate (p<0.01) to effectively improve cell viability. Moreover, the combined influence of both the strain stimulation intensity and number of stimulations on the fibroblast survival rate significantly differed (p<0.05). Strain intensity also significantly altered fibroblast protein expression between the three groups (p<0.0001). Cluster analysis showed that the medium-intensity strain stimulation posed the maximum influence on protein expression.ConclusionThe difference in cell viability and protein expression of the connective tissue fibroblast during the in vitro strain process reveals the cytobiological mechanism of basic medicinal mechanical stimulation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Open Life Sciences de Gruyter

Strain Stimulations with Different Intensities on Fibroblast Viability and Protein Expression

Loading next page...
 
/lp/degruyter/strain-stimulations-with-different-intensities-on-fibroblast-viability-JMeKoTwty0
Publisher
De Gruyter Open
Copyright
© 2017 Ying Jia et al.
ISSN
2391-5412
eISSN
2391-5412
D.O.I.
10.1515/biol-2017-0033
Publisher site
See Article on Publisher Site

Abstract

AbstractBackgroundMechanical stimulation via acupuncture and tuina massage triggers various cell responses. This study aims to understand these cellular bio-physical mechanisms by investigating the effect of different stimulation intensities on cell viability and protein expression.MethodologyConnective tissue fibroblasts were cultured in vitro. Three varying intensities of mechanical strain stimulation were applied to the cells, either once or three times and compared with non-stimulated controls. Changes in fibroblast viability and fibroblast protein expression were observed.ResultsStrain stimulation intensity significantly increased fibroblast cell survival rate (p<0.01) to effectively improve cell viability. Moreover, the combined influence of both the strain stimulation intensity and number of stimulations on the fibroblast survival rate significantly differed (p<0.05). Strain intensity also significantly altered fibroblast protein expression between the three groups (p<0.0001). Cluster analysis showed that the medium-intensity strain stimulation posed the maximum influence on protein expression.ConclusionThe difference in cell viability and protein expression of the connective tissue fibroblast during the in vitro strain process reveals the cytobiological mechanism of basic medicinal mechanical stimulation.

Journal

Open Life Sciencesde Gruyter

Published: Oct 23, 2017

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off