IL8 and IL16 levels indicate serum and plasma quality

Olga Kofanova; Estelle Henry; Rocio Aguilar Quesada; Alexandre Bulla; Hector Navarro Linares; Pierre Lescuyer; Kathi Shea; Mars Stone; Gunnel Tybring; Camille Bellora; Fay Betsou

doi:10.1515/cclm-2017-1047

Kofanova, Olga; Henry, Estelle; Aguilar Quesada, Rocio; Bulla, Alexandre; Navarro Linares, Hector; Lescuyer, Pierre; Shea, Kathi; Stone, Mars; Tybring, Gunnel; Bellora, Camille; Betsou, Fay

2018-06-27 00:00:00

AbstractBackground:Longer pre-centrifugation times alter the quality of serum and plasma samples. Markers for such delays in sample processing and hence for the sample quality, have been identified.Methods:Twenty cytokines in serum, EDTA plasma and citrate plasma samples were screened for changes in concentration induced by extended blood pre-centrifugation delays at room temperature. The two cytokines that showed the largest changes were further validated for their “diagnostic performance” in identifying serum or plasma samples with extended pre-centrifugation times.Results:In this study, using R&D Systems ELISA kits, EDTA plasma samples and serum samples with a pre-centrifugation delay longer than 24 h had an IL16 concentration higher than 313 pg/mL, and an IL8 concentration higher than 125 pg/mL, respectively. EDTA plasma samples with a pre-centrifugation delay longer than 48 h had an IL16 concentration higher than 897 pg/mL, citrate plasma samples had an IL8 concentration higher than 21.5 pg/mL and serum samples had an IL8 concentration higher than 528 pg/mL.Conclusions:These robust and accurate tools, based on simple and commercially available ELISA assays can greatly facilitate qualification of serum and plasma legacy collections with undocumented pre-analytics.

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Clinical Chemistry and Laboratory Medicine (CCLM) de Gruyter

http://www.deepdyve.com/lp/de-gruyter/il8-and-il16-levels-indicate-serum-and-plasma-quality-pcSv9c9SSn

IL8 and IL16 levels indicate serum and plasma quality

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IL8 and IL16 levels indicate serum and plasma quality

IL8 and IL16 levels indicate serum and plasma quality

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