IL8 and IL16 levels indicate serum and plasma quality

Olga Kofanova; Estelle Henry; Rocio Aguilar Quesada; Alexandre Bulla; Hector Navarro Linares; Pierre Lescuyer; Kathi Shea; Mars Stone; Gunnel Tybring; Camille Bellora; Fay Betsou

doi:10.1515/cclm-2017-1047

Kofanova, Olga; Henry, Estelle; Aguilar Quesada, Rocio; Bulla, Alexandre; Navarro Linares, Hector; Lescuyer, Pierre; Shea, Kathi; Stone, Mars; Tybring, Gunnel; Bellora, Camille; Betsou, Fay

2018-06-27 00:00:00

AbstractBackground:Longer pre-centrifugation times alter the quality of serum and plasma samples. Markers for such delays in sample processing and hence for the sample quality, have been identified.Methods:Twenty cytokines in serum, EDTA plasma and citrate plasma samples were screened for changes in concentration induced by extended blood pre-centrifugation delays at room temperature. The two cytokines that showed the largest changes were further validated for their “diagnostic performance” in identifying serum or plasma samples with extended pre-centrifugation times.Results:In this study, using R&D Systems ELISA kits, EDTA plasma samples and serum samples with a pre-centrifugation delay longer than 24 h had an IL16 concentration higher than 313 pg/mL, and an IL8 concentration higher than 125 pg/mL, respectively. EDTA plasma samples with a pre-centrifugation delay longer than 48 h had an IL16 concentration higher than 897 pg/mL, citrate plasma samples had an IL8 concentration higher than 21.5 pg/mL and serum samples had an IL8 concentration higher than 528 pg/mL.Conclusions:These robust and accurate tools, based on simple and commercially available ELISA assays can greatly facilitate qualification of serum and plasma legacy collections with undocumented pre-analytics.

http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Clinical Chemistry and Laboratory Medicine (CCLM) de Gruyter

http://www.deepdyve.com/lp/de-gruyter/il8-and-il16-levels-indicate-serum-and-plasma-quality-pcSv9c9SSn

IL8 and IL16 levels indicate serum and plasma quality

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”
Daniel C.

“Whoa! It’s like Spotify but for academic articles.”
@Phil_Robichaud

“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”
@deepthiw

“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”
@JoseServera

‹ ›

	DeepDyve Freelancer	DeepDyve Pro
Price	FREE	$49/month $360/year
Save searches from Google Scholar, PubMed
Create folders to organize your research
Export folders, citations
Read DeepDyve articles	Abstract access only	Unlimited access to over 18 million full-text articles
Print		20 pages / month
PDF Discount		20% off
	Sign up for free	Start 14 day Free Trial

IL8 and IL16 levels indicate serum and plasma quality

IL8 and IL16 levels indicate serum and plasma quality

You’re reading a free preview. Subscribe to read the entire article.