Evaluation of an automated commercial ELISA method for calprotectin determination in pleural fluid

Evaluation of an automated commercial ELISA method for calprotectin determination in pleural fluid To the Editor,Malignancy is one of the most common causes of exudative pleural effusion (PE) [1], and the discrimination between benign PE (BPE) and PE of malignant origin (MPE) remains a diagnostic challenge [2]. The initial diagnostic approach for exudative PE includes semi-invasive methods such as thoracocentesis, allowing the cytological, microbiological, and biochemical examination of the fluid [3]. Among these, the cytological analysis of the pleural fluid (PF) constitutes the most common method for establishing malignancy [3, 4]. Unfortunately, when a definite diagnosis is not reached, more invasive confirmatory methods result necessary. Moreover, since more than half of the patients with MPE die within 6 months after diagnosis, the correct and rapid diagnosis of benign/malignant PE is mandatory to accomplish an adequate treatment and management of the patient. Thus, non-invasive markers that can be measured in PF are of great interest.In a previous study, we demonstrated that calprotectin, a heterodimeric complex of the proteins S100A8 and S100A9, could be measured in PF using the Hycult assay kit (Biotech), which is useful for discriminating between MPE and BPE (calprotectin levels are higher in BPE) [5]. Several commercial calprotectin ELISA tests are available and established in clinic for the measurement of http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine (CCLM) de Gruyter

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Publisher
De Gruyter
Copyright
©2017 Walter de Gruyter GmbH, Berlin/Boston
ISSN
1437-4331
eISSN
1437-4331
D.O.I.
10.1515/cclm-2016-0884
Publisher site
See Article on Publisher Site

Abstract

To the Editor,Malignancy is one of the most common causes of exudative pleural effusion (PE) [1], and the discrimination between benign PE (BPE) and PE of malignant origin (MPE) remains a diagnostic challenge [2]. The initial diagnostic approach for exudative PE includes semi-invasive methods such as thoracocentesis, allowing the cytological, microbiological, and biochemical examination of the fluid [3]. Among these, the cytological analysis of the pleural fluid (PF) constitutes the most common method for establishing malignancy [3, 4]. Unfortunately, when a definite diagnosis is not reached, more invasive confirmatory methods result necessary. Moreover, since more than half of the patients with MPE die within 6 months after diagnosis, the correct and rapid diagnosis of benign/malignant PE is mandatory to accomplish an adequate treatment and management of the patient. Thus, non-invasive markers that can be measured in PF are of great interest.In a previous study, we demonstrated that calprotectin, a heterodimeric complex of the proteins S100A8 and S100A9, could be measured in PF using the Hycult assay kit (Biotech), which is useful for discriminating between MPE and BPE (calprotectin levels are higher in BPE) [5]. Several commercial calprotectin ELISA tests are available and established in clinic for the measurement of

Journal

Clinical Chemistry and Laboratory Medicine (CCLM)de Gruyter

Published: Jul 26, 2017

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