To the Editor,Urinary aldosterone quantitation has an important but small role to play in the investigation of primary aldosteronism (PA) and less commonly, for secondary aldosteronism and hypoaldosteronism. It is most often used following the oral sodium loading challenge as a confirmatory test for diagnosis of PA .Until recently, most laboratories were offering radioimmunoassay (RIA) detection of urinary aldosterone. A smaller number of laboratories have developed liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods which are expected to be superior to immunoassays from the perspective of analytical specificity. Two automated competitive chemiluminescent immunoassays (CLIA) for the quantitative determination of aldosterone in plasma, serum, and urine samples have become available. The CLIA assays can potentially improve turn-around time and reliability for urinary aldosterone measurement without the use of radiotracers. For the LIAISON assay (Diasorin, Saluggia, Italy), sample preparation requires acid hydrolysis for conversion of metabolites such as aldosterone-18-oxo-glucuronide to aldosterone. While this sample-preparation procedure is manual, the remainder of the procedure is automated, reducing the number of steps compared to RIA. Here, we describe the analytical evaluation for the urinary aldosterone method on the DiaSorin-LIAISON platform and provide patient comparison data to conventional RIA and LC-MS/MS methods.Urine samples were subject to acid
Clinical Chemistry and Laboratory Medicine (CCLM) – de Gruyter
Published: Jul 26, 2017
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