Effects of apixaban on prothrombin time, activated partial thromboplastin time and anti-Xa assays: a European survey

Effects of apixaban on prothrombin time, activated partial thromboplastin time and anti-Xa... To the Editor,Direct FXa inhibitor apixaban affects routine coagulation assays of PT/INR and APTT to some extent. Generally, increasing apixaban concentrations only modestly prolongs coagulation times [1]. Yet, responses are reagent-dependent and laboratories need to recognize the sensitivities of their reagents. Previously, we conducted a survey in 73 and 22 European laboratories with dabigatran and rivaroxaban, respectively, to assess the anticoagulant effects on routine coagulation assays [2].Here, we repeated the survey using apixaban. In September 2015, an EQA organization Labquality Ltd (Finland) sent lyophilized apixaban samples (kindly donated by Pfizer Finland) to 28 European laboratories participating in an anti-Xa EQA round (Finland, Denmark, Estonia, Greece, Iceland, Ireland, Norway) spiked in different concentrations: 80 ng/mL (Diagnostica Stago), 120 ng/mL (Technoclone), 270 ng/mL (Stago), and 300 ng/mL (Technoclone). Laboratories were instructed to reconstitute the samples with distilled water and incubate for 30 min at room temperature, before analysis. They were instructed to report INR, APTT and anti-Xa (with both heparin and apixaban calibration), where available.Twenty-one laboratories reported results (16 Finland, one Estonia, one Greece, one Iceland, one Ireland, one Norway). The laboratories included 10 university centers (five in Finland), with other participants being regional laboratories. PT results were reported as activity http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine (CCLM) de Gruyter

Effects of apixaban on prothrombin time, activated partial thromboplastin time and anti-Xa assays: a European survey

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Publisher
De Gruyter
Copyright
©2017 Walter de Gruyter GmbH, Berlin/Boston
ISSN
1437-4331
eISSN
1437-4331
D.O.I.
10.1515/cclm-2016-0742
Publisher site
See Article on Publisher Site

Abstract

To the Editor,Direct FXa inhibitor apixaban affects routine coagulation assays of PT/INR and APTT to some extent. Generally, increasing apixaban concentrations only modestly prolongs coagulation times [1]. Yet, responses are reagent-dependent and laboratories need to recognize the sensitivities of their reagents. Previously, we conducted a survey in 73 and 22 European laboratories with dabigatran and rivaroxaban, respectively, to assess the anticoagulant effects on routine coagulation assays [2].Here, we repeated the survey using apixaban. In September 2015, an EQA organization Labquality Ltd (Finland) sent lyophilized apixaban samples (kindly donated by Pfizer Finland) to 28 European laboratories participating in an anti-Xa EQA round (Finland, Denmark, Estonia, Greece, Iceland, Ireland, Norway) spiked in different concentrations: 80 ng/mL (Diagnostica Stago), 120 ng/mL (Technoclone), 270 ng/mL (Stago), and 300 ng/mL (Technoclone). Laboratories were instructed to reconstitute the samples with distilled water and incubate for 30 min at room temperature, before analysis. They were instructed to report INR, APTT and anti-Xa (with both heparin and apixaban calibration), where available.Twenty-one laboratories reported results (16 Finland, one Estonia, one Greece, one Iceland, one Ireland, one Norway). The laboratories included 10 university centers (five in Finland), with other participants being regional laboratories. PT results were reported as activity

Journal

Clinical Chemistry and Laboratory Medicine (CCLM)de Gruyter

Published: Jul 26, 2017

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