Effect of haemolysis on the determination of CCL17/thymus and activation-regulated chemokine (TARC) and CCL22/macrophage-derived chemokine (MDC)

Effect of haemolysis on the determination of CCL17/thymus and activation-regulated chemokine... To the Editor,Recently, studies have suggested that levels of the chemokines CCL17/thymus and activation-regulated chemokine (TARC) and CCL22/macrophage-derived chemokine (MDC) in umbilical cord blood may be predictive of whether infants will develop atopic dermatitis during infancy [1, 2]. Whilst preparing cord blood serum samples from an infant cohort study prior to TARC and MDC analysis we noted that 92 out of 717 samples (12.8%) were visibly haemolysed to a variable degree. Insert brochures from the ELISA kits to be used for the chemokines measurement (Quantikine ELISA kits, R&D Systems Inc., Minneapolis, MN, USA) mention haemoglobin as an interfering factor for TARC analysis, but no mention for this for MDC analysis. This prompted us to separately investigate whether haemolysis affects TARC and/or MDC levels using these ELISA kits.A number of different techniques exist for generating haemolysates for haemolysis interference studies [3]. We chose the recommendation by Lippi [3] to serially aspirate anticoagulated blood samples through a small needle to obtain plasma samples with varying degrees of haemolysis [4]. This has the advantage of lysing white and red blood cells and platelets, which may contain the compounds being tested for in different levels to what is found in serum or plasma. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine (CCLM) de Gruyter

Effect of haemolysis on the determination of CCL17/thymus and activation-regulated chemokine (TARC) and CCL22/macrophage-derived chemokine (MDC)

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Publisher
De Gruyter
Copyright
©2018 Walter de Gruyter GmbH, Berlin/Boston
ISSN
1437-4331
eISSN
1437-4331
D.O.I.
10.1515/cclm-2017-0776
Publisher site
See Article on Publisher Site

Abstract

To the Editor,Recently, studies have suggested that levels of the chemokines CCL17/thymus and activation-regulated chemokine (TARC) and CCL22/macrophage-derived chemokine (MDC) in umbilical cord blood may be predictive of whether infants will develop atopic dermatitis during infancy [1, 2]. Whilst preparing cord blood serum samples from an infant cohort study prior to TARC and MDC analysis we noted that 92 out of 717 samples (12.8%) were visibly haemolysed to a variable degree. Insert brochures from the ELISA kits to be used for the chemokines measurement (Quantikine ELISA kits, R&D Systems Inc., Minneapolis, MN, USA) mention haemoglobin as an interfering factor for TARC analysis, but no mention for this for MDC analysis. This prompted us to separately investigate whether haemolysis affects TARC and/or MDC levels using these ELISA kits.A number of different techniques exist for generating haemolysates for haemolysis interference studies [3]. We chose the recommendation by Lippi [3] to serially aspirate anticoagulated blood samples through a small needle to obtain plasma samples with varying degrees of haemolysis [4]. This has the advantage of lysing white and red blood cells and platelets, which may contain the compounds being tested for in different levels to what is found in serum or plasma.

Journal

Clinical Chemistry and Laboratory Medicine (CCLM)de Gruyter

Published: Mar 28, 2018

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