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Biomarkers alterations in trapezius muscle after an acute tissue trauma: A human microdialysis study

Biomarkers alterations in trapezius muscle after an acute tissue trauma: A human microdialysis study 184 Scientific presentations at the 2017 Annual Meeting / Scandinavian Journal of Pain 16 (2017) 165–188 AQP4 expression in trigeminal neurons and SGCs under normal and macro mean, and macro max was found. The micro mean was inflammatory conditions relevant to craniofacial pain conditions. chosen as a proxy for the amount of synovitis. A multiple lin- Methods: Rat trigeminal ganglia (TG) were isolated from adult ear regression analysis, correcting for baseline values of BMI, age, male Sprague-Dawley rats subjected to a model of trigeminal smoking, point pressure thresholds (PPTs) and KOOS pain, showed that the synovitis measurement at baseline was significant for the inflammation evoked by unilateral complete Freund’s adjuvant (CFA) injection in temporomandibular joint. Immunohistochem- change in KOOS pain with an effect of 22.22 (p = 0.0498). Hence, the istry was performed on TG sections of CFA-treated animals. NeuN more synovitis at baseline, the higher the follow-up KOOS Pain was and GS markers were used for identification of neurons and SGCs, compared to baseline KOOS Pain (an improvement). The standard respectively. AQP4 expression was investigated in both ipsilateral deviation (SD) of the synovitis measure was 0.42, hence a change and contralateral TG sections. The study protocol was approved by of 1 SD in synovitis gave an increase of 9 in KOOS Pain at follow-up the local ethics committee. compared to at baseline. Results: Co-localization of NeuN-AQP4 and GS-AQP4 were Conclusions: These preliminary results indicate that the syn- identified in both ipsi and contralateral trigeminal ganglia of ovitis score attained during operation is positively correlated with the CFA-treated rats. However, we did not detect any difference improvement in KOOS-pain post operatively. between the ipsi- and contralateral side in terms of alteration in AQP4 receptor expression. http://dx.doi.org/10.1016/j.sjpain.2017.04.056 Conclusions: AQP4 was expressed both on trigeminal neurons and SGCs and CFA did not cause a remarkable change in AQP4 Biomarkers alterations in trapezius muscle expression, when ipsilateral and contralateral TG of the test animals after an acute tissue trauma: A human was compared. Previously, it has been shown that in a neuro- microdialysis study pathic pain model no difference is detectable between wild type a,∗ a b L.B. Sørensen , P. Gazerani , K. Wåhlén ,N. and AQP4-deficient mice, for mechanical and thermal perception; b b b Ghafouri , B. Gerdle , B. Ghafouri however, in formalin pain model AQP4-deficient mice have higher thermal pain thresholds. Further investigation is required to clarify Center for Neuroplasticity and Pain (CNAP), SMI, role of AQP4 in pain. Department of Health Science and Technology, Aalborg University, Aalborg, Denmark http://dx.doi.org/10.1016/j.sjpain.2017.04.055 Pain and Rehabilitation Centre, and Department of Medical and Health Sciences, Linköping University, Preoperative synovitis in knee osteoarthritis is Linköping, Sweden predictive for pain 1 year after total knee E-mail address: lbs@hst.aau.dk (L.B. Sørensen). arthroplasty Aims: Alteration in muscle milieu has been proposed as one of the main contributors underlying chronic musculoskeletal pain a,b,c,d,∗ Jonathan Vela , Kristian Kjær (CMP). Microdialysis (MD) provides real-time information on a,b,c,d a,b,c,d Petersen , Lars Arendt-Nielsen , Mikkel release of pain and metabolic biomarkers in muscle. However, a,b,c,d a,b,c,d Meyer Andersen , Ole Simonsen insertion of MD probe causes a local tissue trauma, which may Department of Rheumatology, Aalborg, Denmark affect the tissue milieu. Whether an acute tissue trauma alter pain Centre for Sensory-Motor Interaction, Aalborg, and metabolite biomarkers in trapezius muscle of patients with Denmark CMP is not known. Hence, this study investigated changes in mus- Department of Mathematical Sciences, Aalborg, cle metabolites following MD probe insertion in patients with CMP Denmark in comparison with healthy controls. Department of Orthopaedic Surgery, Aalborg, Methods: Nineteen patients (11 women and 8 men; 41.4 years) Denmark with CMP and 20 pain-free volunteers (10 women and 10 men; E-mail address: j.vela@rn.dk (J. Vela). 36.5 years) were recruited (project approval number: 2013/151- Background: Knee osteoarthritis (KOA) was previously con- 31). Baseline pressure pain thresholds (PPT) at trapezius muscle sidered a degenerative joint disease due to wear and tear. After were obtained bilaterally with a reference point at the tibialis mus- total Knee replacement (TKR) 13–38% of patients develop persis- cle. Pain questionnaires were used for determining levels of anxiety tent post-operative pain. Emerging evidence has shown that the and depression and catastrophizing impact. Interstitial samples etiology of pain in KOA is multifaceted and may involve different were collected from trapezius muscles by aid of MD (20 kDa cut-off) structures including the synovial membrane, musculotendinous during a period of 40 min. Collected dialysates at 2 time-points of structures and neuronal tissue. Current preoperative assessment 20 and 40 min were stored at −70 C until analysis. Concentrations does not take these into account, thus the current study examines of glucose, lactate, pyruvate, glycerol, and glutamate were analyzed flex the role of preoperative synovitis on patient reported outcomes by ISCUSS . P ≤ 0.05 was considered significant. one-year post surgery. Results: No potential case with respect to anxiety, depression or Material and methods: 40 patients with end-stage KOA catastrophizing impact was found. Lower PPTs were found in CMP answered the KOOS questionnaire prior to receiving total knee group (P ≤ 0.05). Significantly lower levels of pyruvate were found replacement surgery. During the procedure synovitis was scored in CMP group at both 20 min (P = 0.003) and 40 min (P = 0.006). systematically at six sites where synovial excision biopsies were Gender-based analysis indicated higher concentrations of gluta- obtained. Macro scoring was based on three parameters (hypertro- mate in female patients with CMP. phy, vascularity and synovitis) on a 0–4 point scale. Micro biopsies Conclusions: This study was first to demonstrate metabolite where also graded based on three parameters (synovial hyperpla- alterations during trauma phase of MD in trapezius muscles of CPM sia, inflammatory infiltration and activation of synovial stroma) but compared with healthy controls. This model proved beneficial for on a 0–3 point scale. Patients received a follow up KOOS question- investigating pain and metabolic biomarkers during acute phase of naire one-year post surgery. MD. Results: 33 patients completed the KOOS questionnaire at follow-up. A high correlation between the micro mean, micro max, http://dx.doi.org/10.1016/j.sjpain.2017.04.057 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Scandinavian Journal of Pain de Gruyter

Biomarkers alterations in trapezius muscle after an acute tissue trauma: A human microdialysis study

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Publisher
de Gruyter
Copyright
© 2017 Scandinavian Association for the Study of Pain
ISSN
1877-8860
eISSN
1877-8879
DOI
10.1016/j.sjpain.2017.04.057
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Abstract

184 Scientific presentations at the 2017 Annual Meeting / Scandinavian Journal of Pain 16 (2017) 165–188 AQP4 expression in trigeminal neurons and SGCs under normal and macro mean, and macro max was found. The micro mean was inflammatory conditions relevant to craniofacial pain conditions. chosen as a proxy for the amount of synovitis. A multiple lin- Methods: Rat trigeminal ganglia (TG) were isolated from adult ear regression analysis, correcting for baseline values of BMI, age, male Sprague-Dawley rats subjected to a model of trigeminal smoking, point pressure thresholds (PPTs) and KOOS pain, showed that the synovitis measurement at baseline was significant for the inflammation evoked by unilateral complete Freund’s adjuvant (CFA) injection in temporomandibular joint. Immunohistochem- change in KOOS pain with an effect of 22.22 (p = 0.0498). Hence, the istry was performed on TG sections of CFA-treated animals. NeuN more synovitis at baseline, the higher the follow-up KOOS Pain was and GS markers were used for identification of neurons and SGCs, compared to baseline KOOS Pain (an improvement). The standard respectively. AQP4 expression was investigated in both ipsilateral deviation (SD) of the synovitis measure was 0.42, hence a change and contralateral TG sections. The study protocol was approved by of 1 SD in synovitis gave an increase of 9 in KOOS Pain at follow-up the local ethics committee. compared to at baseline. Results: Co-localization of NeuN-AQP4 and GS-AQP4 were Conclusions: These preliminary results indicate that the syn- identified in both ipsi and contralateral trigeminal ganglia of ovitis score attained during operation is positively correlated with the CFA-treated rats. However, we did not detect any difference improvement in KOOS-pain post operatively. between the ipsi- and contralateral side in terms of alteration in AQP4 receptor expression. http://dx.doi.org/10.1016/j.sjpain.2017.04.056 Conclusions: AQP4 was expressed both on trigeminal neurons and SGCs and CFA did not cause a remarkable change in AQP4 Biomarkers alterations in trapezius muscle expression, when ipsilateral and contralateral TG of the test animals after an acute tissue trauma: A human was compared. Previously, it has been shown that in a neuro- microdialysis study pathic pain model no difference is detectable between wild type a,∗ a b L.B. Sørensen , P. Gazerani , K. Wåhlén ,N. and AQP4-deficient mice, for mechanical and thermal perception; b b b Ghafouri , B. Gerdle , B. Ghafouri however, in formalin pain model AQP4-deficient mice have higher thermal pain thresholds. Further investigation is required to clarify Center for Neuroplasticity and Pain (CNAP), SMI, role of AQP4 in pain. Department of Health Science and Technology, Aalborg University, Aalborg, Denmark http://dx.doi.org/10.1016/j.sjpain.2017.04.055 Pain and Rehabilitation Centre, and Department of Medical and Health Sciences, Linköping University, Preoperative synovitis in knee osteoarthritis is Linköping, Sweden predictive for pain 1 year after total knee E-mail address: lbs@hst.aau.dk (L.B. Sørensen). arthroplasty Aims: Alteration in muscle milieu has been proposed as one of the main contributors underlying chronic musculoskeletal pain a,b,c,d,∗ Jonathan Vela , Kristian Kjær (CMP). Microdialysis (MD) provides real-time information on a,b,c,d a,b,c,d Petersen , Lars Arendt-Nielsen , Mikkel release of pain and metabolic biomarkers in muscle. However, a,b,c,d a,b,c,d Meyer Andersen , Ole Simonsen insertion of MD probe causes a local tissue trauma, which may Department of Rheumatology, Aalborg, Denmark affect the tissue milieu. Whether an acute tissue trauma alter pain Centre for Sensory-Motor Interaction, Aalborg, and metabolite biomarkers in trapezius muscle of patients with Denmark CMP is not known. Hence, this study investigated changes in mus- Department of Mathematical Sciences, Aalborg, cle metabolites following MD probe insertion in patients with CMP Denmark in comparison with healthy controls. Department of Orthopaedic Surgery, Aalborg, Methods: Nineteen patients (11 women and 8 men; 41.4 years) Denmark with CMP and 20 pain-free volunteers (10 women and 10 men; E-mail address: j.vela@rn.dk (J. Vela). 36.5 years) were recruited (project approval number: 2013/151- Background: Knee osteoarthritis (KOA) was previously con- 31). Baseline pressure pain thresholds (PPT) at trapezius muscle sidered a degenerative joint disease due to wear and tear. After were obtained bilaterally with a reference point at the tibialis mus- total Knee replacement (TKR) 13–38% of patients develop persis- cle. Pain questionnaires were used for determining levels of anxiety tent post-operative pain. Emerging evidence has shown that the and depression and catastrophizing impact. Interstitial samples etiology of pain in KOA is multifaceted and may involve different were collected from trapezius muscles by aid of MD (20 kDa cut-off) structures including the synovial membrane, musculotendinous during a period of 40 min. Collected dialysates at 2 time-points of structures and neuronal tissue. Current preoperative assessment 20 and 40 min were stored at −70 C until analysis. Concentrations does not take these into account, thus the current study examines of glucose, lactate, pyruvate, glycerol, and glutamate were analyzed flex the role of preoperative synovitis on patient reported outcomes by ISCUSS . P ≤ 0.05 was considered significant. one-year post surgery. Results: No potential case with respect to anxiety, depression or Material and methods: 40 patients with end-stage KOA catastrophizing impact was found. Lower PPTs were found in CMP answered the KOOS questionnaire prior to receiving total knee group (P ≤ 0.05). Significantly lower levels of pyruvate were found replacement surgery. During the procedure synovitis was scored in CMP group at both 20 min (P = 0.003) and 40 min (P = 0.006). systematically at six sites where synovial excision biopsies were Gender-based analysis indicated higher concentrations of gluta- obtained. Macro scoring was based on three parameters (hypertro- mate in female patients with CMP. phy, vascularity and synovitis) on a 0–4 point scale. Micro biopsies Conclusions: This study was first to demonstrate metabolite where also graded based on three parameters (synovial hyperpla- alterations during trauma phase of MD in trapezius muscles of CPM sia, inflammatory infiltration and activation of synovial stroma) but compared with healthy controls. This model proved beneficial for on a 0–3 point scale. Patients received a follow up KOOS question- investigating pain and metabolic biomarkers during acute phase of naire one-year post surgery. MD. Results: 33 patients completed the KOOS questionnaire at follow-up. A high correlation between the micro mean, micro max, http://dx.doi.org/10.1016/j.sjpain.2017.04.057

Journal

Scandinavian Journal of Pain – de Gruyter

Published: Jul 1, 2017

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