Transport Kinetics for the Uptake of ( 3 H)Glycine and ( 3 H)L-Glutamate into Dorsal and Ventral Slices of Rat Spinal Cord

Transport Kinetics for the Uptake of ( 3 H)Glycine and ( 3 H)L-Glutamate into Dorsal and Ventral... Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 1097 -1101, August 1976 Transport Kinetics for the Uptake of [3H]Glycine and [3H]L-Glutamate into Dorsal and Ventral Slices of Rat Spinal Cord Conrad G. HONEGGER, Marianne STEINER and Holger P. VON HAHN Abteilung Neurochemie, Neurologische Universitätsklinik, Basel, Schweiz (Received 5 May 1976) Summary: The uptake of [3H]glycine and [ 3 H]Lglutamate into slices of the dorsal and ventral halves of rat spinal cord taken from the thoracal (T3-9) and lumbar (LI-6) regions was determined in the concentration range 2 - 1 . For both amino acids and in all four tissue pieces (thoracal dorsal and ventral, lumbar dorsal and ventral) biphasic linear regressions were obtained from the Lineweaver-Burk plots, demonstrating saturable transport mechanisms both in the highaffinity and in the low-affinity range. No signifi- cant dorsal-ventral or lumbar-thoracal differences were found for the transport constants Km of both amino acids. For the maximum velocity V similar dorsal-ventral and lumbar-thoracal gradients were found for both glycine and L-glutamate. These results, which are not entirely in agreement with the distribution of the endogenous pools of these amino acids, are critically discussed in view of the proposed function of high affinity uptake at the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png hoppe-seyler's zeitschrift für physiologische chemie de Gruyter

Transport Kinetics for the Uptake of ( 3 H)Glycine and ( 3 H)L-Glutamate into Dorsal and Ventral Slices of Rat Spinal Cord

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Publisher
de Gruyter
Copyright
Copyright © 1976 by the
ISSN
0018-4888
eISSN
1437-4315
DOI
10.1515/bchm2.1976.357.2.1097
Publisher site
See Article on Publisher Site

Abstract

Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 1097 -1101, August 1976 Transport Kinetics for the Uptake of [3H]Glycine and [3H]L-Glutamate into Dorsal and Ventral Slices of Rat Spinal Cord Conrad G. HONEGGER, Marianne STEINER and Holger P. VON HAHN Abteilung Neurochemie, Neurologische Universitätsklinik, Basel, Schweiz (Received 5 May 1976) Summary: The uptake of [3H]glycine and [ 3 H]Lglutamate into slices of the dorsal and ventral halves of rat spinal cord taken from the thoracal (T3-9) and lumbar (LI-6) regions was determined in the concentration range 2 - 1 . For both amino acids and in all four tissue pieces (thoracal dorsal and ventral, lumbar dorsal and ventral) biphasic linear regressions were obtained from the Lineweaver-Burk plots, demonstrating saturable transport mechanisms both in the highaffinity and in the low-affinity range. No signifi- cant dorsal-ventral or lumbar-thoracal differences were found for the transport constants Km of both amino acids. For the maximum velocity V similar dorsal-ventral and lumbar-thoracal gradients were found for both glycine and L-glutamate. These results, which are not entirely in agreement with the distribution of the endogenous pools of these amino acids, are critically discussed in view of the proposed function of high affinity uptake at the

Journal

hoppe-seyler's zeitschrift für physiologische chemiede Gruyter

Published: Jan 1, 1976

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