Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 1487 -1494, November 1976 The Use of 8-Anilino-l-naphthalenesulfonic Acid as a Reporter Group Molecule for Circular Dichroism and Fluorescence Measurements The Effect of Stearic acid and Sodium Dodecylsulfate on the Conformation of Bovine and Human Serum Albumin Walter E. MÜLLER Pharmakologisches Institut der Universität Mainz (Received 30 July 1976) Summary: The fluorescence probe ANS(8-anilino 1-naphthalenesulfonic acid) was employed as a reporter group molecule for circular dichroism and fluorescence measurements in order to investigate the effects of stearic acid and sodium dodecylsulfate on the conformation of bovine and human serum albumin. Each of the four extrinsic CD bands of bound ANS was influenced in a different manner by the addition of stearate and dodecylsulfate. Using the data of the fluorescence measurements and of the circular dichroism measurements it was possible to differentiate the effects of one ligand on both albumins and of both ligands on one albumin more efficiently than would have been Stearate as well as dodecylsulfate displaces ANS possible using one of the two methods alone. from the binding to both albumins. Besides this It is suggested that the use of ANS as a reporter displacement, stearate and dodecylsulfate influgroup
hoppe-seyler's zeitschrift für physiologische chemie – de Gruyter
Published: Jan 1, 1976
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