The Sequence Determination of a Protein in a Micro Scale: The Sequence Analysis of Ribosomal Protein L34 of Escherichia coli

The Sequence Determination of a Protein in a Micro Scale: The Sequence Analysis of Ribosomal... Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 873 - 886, Juni 1976 Robert CHEN Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann, Berlin-Dahlem, Germany (Received 25 March 1976) Summary: The complete amino acid sequence of ribosomal protein L34 has been established by improved micro techniques with 3 mg of the lyophilized protein. The protein was digested with trypsin, thermolysin and chymotrypsin and the resulting peptides were isolated from fingerprints performed on cellulose thin-layer plates. The amino acid sequences of the peptides were determined by the combined micro dansyl-Edman technique using 5 - 1 0 nmol per sample. Aspartic acid and glutamic acid were distinguished from their amides by use of the color reaction of ninhydrin with the respective amino acid phenylthiohydantoins. Sequenzbestimmung eines Proteins im Mikromaßstab: Die Sequenzanalyse des ribosomalen Proteins L34 aus Escherichia coli. Zusammenfassung: Die vollständige Aminosäuresequenz des Ribosomenproteins L34 wurde mit einer bewährten Mikromethode mit 3 mg des lyophilisierten Proteins bestimmt. Das Protein wurde mit Trypsin, Thermolysin und Chymotrypsin verdaut und die entstandenen Peptide wurden mittels Fingerprints an Cellulose-Dünnschichtplatten isoliert. Die Aminosäuresequenzen der einzelnen Peptide wurden mit der kombinierten MikroDansyl-Edman-Methode unter Verwendung von Enzymes: Trypsin (EC 3.4.21.4); thermolysin (EC 3.4.24.4); -chymotrypsin (EC 3.4.21.1); carboxypeptidase A, peptidyl-L-amino acid http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png hoppe-seyler's zeitschrift für physiologische chemie de Gruyter

The Sequence Determination of a Protein in a Micro Scale: The Sequence Analysis of Ribosomal Protein L34 of Escherichia coli

Loading next page...
 
/lp/de-gruyter/the-sequence-determination-of-a-protein-in-a-micro-scale-the-sequence-2PFYWZElHF
Publisher
de Gruyter
Copyright
Copyright © 1976 by the
ISSN
0018-4888
eISSN
1437-4315
DOI
10.1515/bchm2.1976.357.1.873
Publisher site
See Article on Publisher Site

Abstract

Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 873 - 886, Juni 1976 Robert CHEN Max-Planck-Institut für Molekulare Genetik, Abt. Wittmann, Berlin-Dahlem, Germany (Received 25 March 1976) Summary: The complete amino acid sequence of ribosomal protein L34 has been established by improved micro techniques with 3 mg of the lyophilized protein. The protein was digested with trypsin, thermolysin and chymotrypsin and the resulting peptides were isolated from fingerprints performed on cellulose thin-layer plates. The amino acid sequences of the peptides were determined by the combined micro dansyl-Edman technique using 5 - 1 0 nmol per sample. Aspartic acid and glutamic acid were distinguished from their amides by use of the color reaction of ninhydrin with the respective amino acid phenylthiohydantoins. Sequenzbestimmung eines Proteins im Mikromaßstab: Die Sequenzanalyse des ribosomalen Proteins L34 aus Escherichia coli. Zusammenfassung: Die vollständige Aminosäuresequenz des Ribosomenproteins L34 wurde mit einer bewährten Mikromethode mit 3 mg des lyophilisierten Proteins bestimmt. Das Protein wurde mit Trypsin, Thermolysin und Chymotrypsin verdaut und die entstandenen Peptide wurden mittels Fingerprints an Cellulose-Dünnschichtplatten isoliert. Die Aminosäuresequenzen der einzelnen Peptide wurden mit der kombinierten MikroDansyl-Edman-Methode unter Verwendung von Enzymes: Trypsin (EC 3.4.21.4); thermolysin (EC 3.4.24.4); -chymotrypsin (EC 3.4.21.1); carboxypeptidase A, peptidyl-L-amino acid

Journal

hoppe-seyler's zeitschrift für physiologische chemiede Gruyter

Published: Jan 1, 1976

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off