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The Enzymatic Microestimation of Urea

The Enzymatic Microestimation of Urea Z. klin. Chem. u. klin. Biochcm. 7. Jg,, S. 32--33, Januar 1969 Manoukian and Fawaz: The Enzymatic Microestimation of Urea By E. MANOUKIAN and G. FAWAZ Department of Pharmacology, School of Medicine American University of Beirut, Beirut, Lebanon (Eingegangen am 21. August 1968) An enzymatic micromethod for estimating urea in serum and urine is described. Urea is first converted by urease into ammonia which is then estimated by the reaction with -ketoglutarate catalysed by glutamic dehydrogenase. No deproteinization or dialysis of the serum is required. Es wird eine enzymatische Mikromethode f r die Bestimmung von Harnstoff in Serum und Urin beschrieben. Nach Hydrolyse mit Urease (Urea Amidohydrolase EC 3.5.1.5) wird das Ammoniak mit Glutamatdehydrogenase (L-Glutamat: NAD Oxydoreduktase, desaminierend, EC 1.4.1.2) bestimmt. Enteiwei ung oder Dialyse des Serums ist nicht erforderlich. FAWAZ and v. DAHL (1) and KIRSTEN, GEREZ and KIRSTEN (2) published an enzymatic method for the estimation of ammonia in tissues and body fluids. MONDZAC, EHRLICH and SEEGMILLER (3), apparently unaware of the above publications, also described the same procedure. The method is based on the reaction: NH+ + H+ + NADH + a-ketoglutarate glutamic dehydrogenase^ L.glutamate + NAD+ Since the reaction goes to completion under experimental http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

The Enzymatic Microestimation of Urea

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1969.7.1.32
Publisher site
See Article on Publisher Site

Abstract

Z. klin. Chem. u. klin. Biochcm. 7. Jg,, S. 32--33, Januar 1969 Manoukian and Fawaz: The Enzymatic Microestimation of Urea By E. MANOUKIAN and G. FAWAZ Department of Pharmacology, School of Medicine American University of Beirut, Beirut, Lebanon (Eingegangen am 21. August 1968) An enzymatic micromethod for estimating urea in serum and urine is described. Urea is first converted by urease into ammonia which is then estimated by the reaction with -ketoglutarate catalysed by glutamic dehydrogenase. No deproteinization or dialysis of the serum is required. Es wird eine enzymatische Mikromethode f r die Bestimmung von Harnstoff in Serum und Urin beschrieben. Nach Hydrolyse mit Urease (Urea Amidohydrolase EC 3.5.1.5) wird das Ammoniak mit Glutamatdehydrogenase (L-Glutamat: NAD Oxydoreduktase, desaminierend, EC 1.4.1.2) bestimmt. Enteiwei ung oder Dialyse des Serums ist nicht erforderlich. FAWAZ and v. DAHL (1) and KIRSTEN, GEREZ and KIRSTEN (2) published an enzymatic method for the estimation of ammonia in tissues and body fluids. MONDZAC, EHRLICH and SEEGMILLER (3), apparently unaware of the above publications, also described the same procedure. The method is based on the reaction: NH+ + H+ + NADH + a-ketoglutarate glutamic dehydrogenase^ L.glutamate + NAD+ Since the reaction goes to completion under experimental

Journal

Clinical Chemistry and Laboratory Medicine – de Gruyter

Published: Jan 1, 1969

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