Introduction Creatinine, an important analyte particularly for assessment of kidney function, was discovered in 1844 by Max von Pettenkofer (1). About 30 years later, Eur. J. Clin. Chem. Clin. Biochero. / Vol. 31,1993 / No. 5 Max Jaffe (2) determined that under alkaline conditions creatinine forms a red-orange dye with picric acid. As the reaction was non-specific, with a great number of other substances also reacting to form this Carstensen et al.: Development and evaluation of a new reagent carrier for the determination of creatinine dye, creatinine could only be determined in urine. Finally, 1937, after the photometric method had been refined, Dubos & Miller (3) managed to furnish proof of the existence of creatinine in the blood. Because of its simplicity the modified Jaffe reaction is still frequently used today. Its analytical specificity, however, is still not sufficient (4), not even with kinetic monitoring of the reaction. The development of enzymatic methods then paved the way for a specific determination of creatinine. A. W. Wahlefeld et al. (5) in 1972 were one of the first to describe an enzyme-mediated assay. Creatinine was hydrolyzed to creatine using creatinine amidohydrolase1), NADH was then oxidized via the subsequent reaction steps
Clinical Chemistry and Laboratory Medicine – de Gruyter
Published: Jan 1, 1993
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