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TECHNICAL NOTE

TECHNICAL NOTE Introduction Elastase/arproteinase-inhibitor complex is a marker for inflammatory disease activity in patients with rheumatic diseases (1), active Crohn disease (2), postoperative infections after major abdominal surgery (3) and multiple trauma (4) as well as for monitoring therapy (5). The present study was undertaken to verify the performance criteria of the 2 h-test kit versus the 4 litest kit. Tab. 1. Imprecision within-run, between-days and accuracy for the 21l·· and 4 h-test kits of elastase/ai-proteinase-inhibitorenzyme-Hnked-immunosorbent assay (CV = coefficient of variation) Imprecision within run Concen- DiluSample tration tion volume (ml) fog/i) 2 faversion Imprecision between days Accuracy Test series (n) Test series (n) CV (%) Concen- Dilutration tion x (/1) Sample volume (ml) CV (%) 9.1 Found concentration Oig/1) 296.0 300.3 303.7 308.9 309.4 Assigned value (Mg/1) 305.0 1:51 1:51 1 : 51 4 faversion 1:51 1:51 1 : 51 139,0 J. Clin. Chem. Clin. Biochem. / Vol. 26,1988 / No. 9 diluted with sample diluent (phosphate, 0.01 mol/1, pH 7.5; NaCl, 0.15 mol/1; EDTA, 20 mmol/1; bovine serum albumin, 1 g/1) (6) in a ratio from 1/100 to 1/1500. Statistical analysis For evaluation of results, imprecision within-run and betweendays, coefficient of correlation (r), standard principle component analysis and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

TECHNICAL NOTE

Clinical Chemistry and Laboratory Medicine , Volume 26 (9) – Jan 1, 1988
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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1988.26.9.583
Publisher site
See Article on Publisher Site

Abstract

Introduction Elastase/arproteinase-inhibitor complex is a marker for inflammatory disease activity in patients with rheumatic diseases (1), active Crohn disease (2), postoperative infections after major abdominal surgery (3) and multiple trauma (4) as well as for monitoring therapy (5). The present study was undertaken to verify the performance criteria of the 2 h-test kit versus the 4 litest kit. Tab. 1. Imprecision within-run, between-days and accuracy for the 21l·· and 4 h-test kits of elastase/ai-proteinase-inhibitorenzyme-Hnked-immunosorbent assay (CV = coefficient of variation) Imprecision within run Concen- DiluSample tration tion volume (ml) fog/i) 2 faversion Imprecision between days Accuracy Test series (n) Test series (n) CV (%) Concen- Dilutration tion x (/1) Sample volume (ml) CV (%) 9.1 Found concentration Oig/1) 296.0 300.3 303.7 308.9 309.4 Assigned value (Mg/1) 305.0 1:51 1:51 1 : 51 4 faversion 1:51 1:51 1 : 51 139,0 J. Clin. Chem. Clin. Biochem. / Vol. 26,1988 / No. 9 diluted with sample diluent (phosphate, 0.01 mol/1, pH 7.5; NaCl, 0.15 mol/1; EDTA, 20 mmol/1; bovine serum albumin, 1 g/1) (6) in a ratio from 1/100 to 1/1500. Statistical analysis For evaluation of results, imprecision within-run and betweendays, coefficient of correlation (r), standard principle component analysis and

Journal

Clinical Chemistry and Laboratory Medicine – de Gruyter

Published: Jan 1, 1988

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