Biol. Chem. Hoppe-Seyler Vol. 374, pp. 1057-1062, November 1993 Zhiping CHEN and Bahram HEMMASI Institut für Organische Chemie der Universität Tübingen, 72076Tübingen, Germany (Received 29 June 1992 / 26 July 1993) Dedicated to Professor Ernst Bayer on the occasion of his 65th birthday Summary: A nona- and an undecapeptide corresponding to phytochelatins with the general structure H-[y-Glu-Cys],,-Gly-OH were each synthesized by the continuous flow solid phase method using two different methodologies. Fmoc-amino acid derivatives were used as precursors, and two different HaN-POEPS supports were employed. Different procedures were used to remove Acm protecting groups from Cys residues. In a second synthesis, Acm groups were removed before cleavage of the peptides from the polymer supports.The partially protected peptides of the first synthesis were purified by preparative HPLC. The purity and identity of all the synthesized peptides were verified by analytical HPLC and IS-MS and in some cases by amino acid analysis. Key terms: Continuous flow synthesis, phytochelatins, heavy metal toxicity, POE-PS. Phytochelatins (PCs) are small peptides with the general structure H-[y-Glu-Cys-],rGly-OH (n = 2-ll)[UJ which play a major role in detoxification of heavy metals in plants, fungi and algae. Upon exposure to heavy metal ions, such as Cd2@, Zn2®,
Biological Chemistry Hoppe-Seyler – de Gruyter
Published: Jan 1, 1993
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