Synthesis of Phytochelatins by the Continuous Flow Solid Phase Procedure

Synthesis of Phytochelatins by the Continuous Flow Solid Phase Procedure Biol. Chem. Hoppe-Seyler Vol. 374, pp. 1057-1062, November 1993 Zhiping CHEN and Bahram HEMMASI Institut für Organische Chemie der Universität Tübingen, 72076Tübingen, Germany (Received 29 June 1992 / 26 July 1993) Dedicated to Professor Ernst Bayer on the occasion of his 65th birthday Summary: A nona- and an undecapeptide corresponding to phytochelatins with the general structure H-[y-Glu-Cys],,-Gly-OH were each synthesized by the continuous flow solid phase method using two different methodologies. Fmoc-amino acid derivatives were used as precursors, and two different HaN-POEPS supports were employed. Different procedures were used to remove Acm protecting groups from Cys residues. In a second synthesis, Acm groups were removed before cleavage of the peptides from the polymer supports.The partially protected peptides of the first synthesis were purified by preparative HPLC. The purity and identity of all the synthesized peptides were verified by analytical HPLC and IS-MS and in some cases by amino acid analysis. Key terms: Continuous flow synthesis, phytochelatins, heavy metal toxicity, POE-PS. Phytochelatins (PCs) are small peptides with the general structure H-[y-Glu-Cys-],rGly-OH (n = 2-ll)[UJ which play a major role in detoxification of heavy metals in plants, fungi and algae. Upon exposure to heavy metal ions, such as Cd2@, Zn2®, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biological Chemistry Hoppe-Seyler de Gruyter

Synthesis of Phytochelatins by the Continuous Flow Solid Phase Procedure

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Publisher
de Gruyter
Copyright
Copyright © 1993 by the
ISSN
0177-3593
eISSN
1437-4315
DOI
10.1515/bchm3.1993.374.7-12.1057
Publisher site
See Article on Publisher Site

Abstract

Biol. Chem. Hoppe-Seyler Vol. 374, pp. 1057-1062, November 1993 Zhiping CHEN and Bahram HEMMASI Institut für Organische Chemie der Universität Tübingen, 72076Tübingen, Germany (Received 29 June 1992 / 26 July 1993) Dedicated to Professor Ernst Bayer on the occasion of his 65th birthday Summary: A nona- and an undecapeptide corresponding to phytochelatins with the general structure H-[y-Glu-Cys],,-Gly-OH were each synthesized by the continuous flow solid phase method using two different methodologies. Fmoc-amino acid derivatives were used as precursors, and two different HaN-POEPS supports were employed. Different procedures were used to remove Acm protecting groups from Cys residues. In a second synthesis, Acm groups were removed before cleavage of the peptides from the polymer supports.The partially protected peptides of the first synthesis were purified by preparative HPLC. The purity and identity of all the synthesized peptides were verified by analytical HPLC and IS-MS and in some cases by amino acid analysis. Key terms: Continuous flow synthesis, phytochelatins, heavy metal toxicity, POE-PS. Phytochelatins (PCs) are small peptides with the general structure H-[y-Glu-Cys-],rGly-OH (n = 2-ll)[UJ which play a major role in detoxification of heavy metals in plants, fungi and algae. Upon exposure to heavy metal ions, such as Cd2@, Zn2®,

Journal

Biological Chemistry Hoppe-Seylerde Gruyter

Published: Jan 1, 1993

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