Introduction Differences among arginases (,-arginine amidinohydrolase, EC 22.214.171.124) from various tissues were noted a long time ago when it was shown that the conditions for measuring arginase activity in one tissue were not equally suitable for arginase tests in another tissue (1). Differences in the pH activity curves (2) and immunochemical properties were also noted (3). Using electrophoretic (4) and Chromatographie techniques two isoenzymes of the mammary gland have been found (4, 5, 6), while the results concerning the number of liver and erythrocyte arginase isoenzymes are not consistent (4, 5, 6, 7, 8). This paper deals with the technique set up in our laboratory for the separation of arginase isoenzymes and with the results obtained with arginase from various human tissues. Material and Methods Tissues were obtained within six hours post mortem. They were washed with saline, freed of connective tissue and of fat which is particularly abundant in the mammary gland. Portions of Or 2 g of tissue were homogenized in z Potter Elvehjem hompgenizer in 1 ml of 5 g/1 Triton X-100 in 0.5 mmol/1 MnQ2 solution. The hpmpgenates were centrifuged at 14,000 g for 20 minutes the supernatants lypphilised and kept dry at +
Clinical Chemistry and Laboratory Medicine – de Gruyter
Published: Jan 1, 1976
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera