Response of 3T3 Cells on Stimulation of Adenylate Cyclase by Forskolin, Studied by Multinuclear NMR Spectroscopy

Response of 3T3 Cells on Stimulation of Adenylate Cyclase by Forskolin, Studied by Multinuclear... Biol. Chem. Hoppe-Seyler Vol. 374, pp. 211-216, March 1993 Ulrich FLÖGEL and Dieter LEIBFRITZ Institut für Organische Chemie, Universität Bremen, Germany (Received 7 December 1992) Summary: Stimulation of 3T3 cells by forskolin (30/xM) led to an increase in cellular cAMP levels, as detected by 31P-NMR, in both PCAextracts and living cells incorporated in basement membrane gel (BMG) threads. At the same time cAMP was exported in large amounts to the extracellular space. The in vivo experiments showed a decrease of more than 50% in both the PCr and ATP levels within 90 min of stimulation with forskolin. Reperfusion with fresh medium without the drug resulted in the full recovery of the phosphorus energy metabolism. A significant increase in phosphocholine (PC) was observed in *H and 31P spectra of the PCA extracts concomitant to the rise in cAMP level. 13C spectra of PCA extracts prepared after forskolin stimulation in the presence of [l-13C]glucose indicated a serious inhibition of glycolysis, which was followed by a disordering of the phosphorus energy metabolism. Our results show a significant imbalance in the metabolic homeostasis during stimulation of 3T3 cells with forskolin. Key terms: Forskolin, cAMP, inhibition of glycolysis, 3T3 cells, in vivo NMR. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biological Chemistry Hoppe-Seyler de Gruyter

Response of 3T3 Cells on Stimulation of Adenylate Cyclase by Forskolin, Studied by Multinuclear NMR Spectroscopy

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Publisher
de Gruyter
Copyright
Copyright © 1993 by the
ISSN
0177-3593
eISSN
1437-4315
DOI
10.1515/bchm3.1993.374.1-6.211
Publisher site
See Article on Publisher Site

Abstract

Biol. Chem. Hoppe-Seyler Vol. 374, pp. 211-216, March 1993 Ulrich FLÖGEL and Dieter LEIBFRITZ Institut für Organische Chemie, Universität Bremen, Germany (Received 7 December 1992) Summary: Stimulation of 3T3 cells by forskolin (30/xM) led to an increase in cellular cAMP levels, as detected by 31P-NMR, in both PCAextracts and living cells incorporated in basement membrane gel (BMG) threads. At the same time cAMP was exported in large amounts to the extracellular space. The in vivo experiments showed a decrease of more than 50% in both the PCr and ATP levels within 90 min of stimulation with forskolin. Reperfusion with fresh medium without the drug resulted in the full recovery of the phosphorus energy metabolism. A significant increase in phosphocholine (PC) was observed in *H and 31P spectra of the PCA extracts concomitant to the rise in cAMP level. 13C spectra of PCA extracts prepared after forskolin stimulation in the presence of [l-13C]glucose indicated a serious inhibition of glycolysis, which was followed by a disordering of the phosphorus energy metabolism. Our results show a significant imbalance in the metabolic homeostasis during stimulation of 3T3 cells with forskolin. Key terms: Forskolin, cAMP, inhibition of glycolysis, 3T3 cells, in vivo NMR.

Journal

Biological Chemistry Hoppe-Seylerde Gruyter

Published: Jan 1, 1993

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