Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Regulated Phosphorylation of the Gal4p Inhibitor Gal80p of Kluyveromyces lactis Revealed by Mutational Analysis

Regulated Phosphorylation of the Gal4p Inhibitor Gal80p of Kluyveromyces lactis Revealed by... Abstract The yeast Gal80 protein inhibits the transcription activation function of Gal4p by physically interacting with the activation domain (Gal4-AD). Gal80p interaction with Gal1p or Gal3p is required to relieve Gal4p inhibition in response to galactose. Gal80p orthologs of Saccharomyces cerevisiae and Kluyveromyces lactis , ScGal80p and KlGal80p, can also inhibit the heterologous Gal4p variants; however, heterologous Gal3p/Gal1p only regulate ScGal80p but not KlGal80p. To compare KlGal80p and ScGal80p, point mutations known to affect ScGal80p function were introduced at corresponding positions in KlGal80p, and Gal4p regulation in vivo and KlGal80p-binding to Gst-Gal1p and Gst-Gal4-AD in vitro were analysed. The in vitro binding properties of the KlGal80p mutants were similar to those of ScGal80p, but two out of four mutants differed in Gal4p regulation. E. g. KlGAL80 s-0 (G302R) but not ScGAL80 s-0 (G301R) alleviates Gal4p inhibition. Possibly, this difference is related to a role of phosphorylation in the regulation of Gal80p function in K. lactis . Wild-type and mutant forms of KlGal80p are shown to be subject to carbon source regulated phosphorylation whereas no evidence for ScGal80p phosphorylation exists. (Hyper-)phosphorylation of KlGal80p is strongly reduced in galactose-containing medium. This reduction requires KlGal1p but no interaction with KlGal4p. The inhibition deficient KlGal80s-0p (G302R) variant is under-phosphorylated. We thus propose that phosphorylation of Gal80p in Kluyveromyces lactis contributes to the regulation of Gal4p mediated transcription. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biological Chemistry de Gruyter

Regulated Phosphorylation of the Gal4p Inhibitor Gal80p of Kluyveromyces lactis Revealed by Mutational Analysis

Biological Chemistry , Volume 380 (4) – Apr 1, 1999

Loading next page...
 
/lp/de-gruyter/regulated-phosphorylation-of-the-gal4p-inhibitor-gal80p-of-4FYqMl5cJZ
Publisher
de Gruyter
Copyright
Copyright © 1999 by the
ISSN
1431-6730
DOI
10.1515/BC.1999.056
pmid
10355628
Publisher site
See Article on Publisher Site

Abstract

Abstract The yeast Gal80 protein inhibits the transcription activation function of Gal4p by physically interacting with the activation domain (Gal4-AD). Gal80p interaction with Gal1p or Gal3p is required to relieve Gal4p inhibition in response to galactose. Gal80p orthologs of Saccharomyces cerevisiae and Kluyveromyces lactis , ScGal80p and KlGal80p, can also inhibit the heterologous Gal4p variants; however, heterologous Gal3p/Gal1p only regulate ScGal80p but not KlGal80p. To compare KlGal80p and ScGal80p, point mutations known to affect ScGal80p function were introduced at corresponding positions in KlGal80p, and Gal4p regulation in vivo and KlGal80p-binding to Gst-Gal1p and Gst-Gal4-AD in vitro were analysed. The in vitro binding properties of the KlGal80p mutants were similar to those of ScGal80p, but two out of four mutants differed in Gal4p regulation. E. g. KlGAL80 s-0 (G302R) but not ScGAL80 s-0 (G301R) alleviates Gal4p inhibition. Possibly, this difference is related to a role of phosphorylation in the regulation of Gal80p function in K. lactis . Wild-type and mutant forms of KlGal80p are shown to be subject to carbon source regulated phosphorylation whereas no evidence for ScGal80p phosphorylation exists. (Hyper-)phosphorylation of KlGal80p is strongly reduced in galactose-containing medium. This reduction requires KlGal1p but no interaction with KlGal4p. The inhibition deficient KlGal80s-0p (G302R) variant is under-phosphorylated. We thus propose that phosphorylation of Gal80p in Kluyveromyces lactis contributes to the regulation of Gal4p mediated transcription.

Journal

Biological Chemistryde Gruyter

Published: Apr 1, 1999

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$499/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month