Hoppe-Seyler's Z, Physiol. Chem. Bd. 357, S. 5l- 56, Januar 1976 Quantitative Determination of Poly(Adenosine Diphosphate Ribose) in Different Hepatic Tissues by an Isotope Dilution Procedure Peter R. STONE, Reinhard BREDEHORST, Margrit KJTTLER, Helgart LENGYEL and Helmuth HILZ Institut für Physiologische Chemie, Universität Hamburg (Received 9 October 1975) Summary: A procedure has been developed for the quantitation of poly(ADP-ribose) in intact tissues. It is based on the dilution of added [3H]poly(ADP-ribose) by the endogenous polymer. 5 - 6 nanomoles protein-bound ADPribose per mg DNA were found in adult and neonatal rat liver, while Zajdela hepatoma cells had significantly lower values. A comparison with mono(ADP-nbose) residues in adult rat liver revealed similar levels of monomeric and polymeric ADP-ribose residues. This means that far more proteins (or acceptor sites on proteins) must be occupied by single ADP-ribose residues than by oligo or poly (ADPribose) chains. While the poly (ADP-ribose) levels of the different tissues do not correlate with the corresponding proliferation rates, the amount of mono (ADPribose) does show a certain Correlation, being low in rapidly growing tissues. Quantitative Bestimmung von poly(Adenosindiphosphat-ribose) in verschiedenen Lebergeweben mittels hotopenverdünnung Zusammenfassung: Eine Methode zur Quantifizierung von poly(ADP-Ribose) in intakten Geweben wird beschrieben. Sie
hoppe-seyler's zeitschrift für physiologische chemie – de Gruyter
Published: Jan 1, 1976
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