Purification and Partial Characterization of Human Pancreatic Elastase

Purification and Partial Characterization of Human Pancreatic Elastase Hoppe-Seyler's Z. PhysioL Chem. Bd. 357, S. 1153 -1161, August 1976 Purification and Partial Characterization of Human Pancreatic Elastase Kjell OHLSSON and Ann-Sofie OLSSON Department of Clinical Chemistry and Surgery, University of Lund, Malmö General Hospital, Malmö, Sweden (Received 6 May 1976) Summary: 1) An elastolytic enzyme has been isolated from human pancreatic juice. The concentration of the enzyme was about 0.6 mg/m/ corresponding to about 10% of the protein concentration of the juice. 2) The purification procedure included chromatography on Sephadex G-25 followed by ion-exchange chromatography on SP-Sephadex C-50 at pH 6.0, affinity chromatography on TrasylolSepharose 4-B and a final ion-exchange chromatography on SP-Sephadex at pH 7.6. The yield was about 50%. 3) The elastase isolated was homogeneous in analytical disc electrophoresis and showed a single protein component with a molecular weight of 26 300 in dodecylsulfate-electrophoresis. There are similarities in the amino acid compositions of human and porcine pancreatic elastases. 4) The human enzyme has a lower activity on elastin than porcine elastase but similar activities on casein and fibrin. The Km value for Boc-Ala-ONp was 5.13 10~*M. The elastase isolated was 98% active, as judged from active site titration. 5) The results of immunodiffusion studies http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png hoppe-seyler's zeitschrift für physiologische chemie de Gruyter

Purification and Partial Characterization of Human Pancreatic Elastase

Loading next page...
 
/lp/de-gruyter/purification-and-partial-characterization-of-human-pancreatic-elastase-KjUr2uPutH
Publisher
de Gruyter
Copyright
Copyright © 1976 by the
ISSN
0018-4888
eISSN
1437-4315
DOI
10.1515/bchm2.1976.357.2.1153
Publisher site
See Article on Publisher Site

Abstract

Hoppe-Seyler's Z. PhysioL Chem. Bd. 357, S. 1153 -1161, August 1976 Purification and Partial Characterization of Human Pancreatic Elastase Kjell OHLSSON and Ann-Sofie OLSSON Department of Clinical Chemistry and Surgery, University of Lund, Malmö General Hospital, Malmö, Sweden (Received 6 May 1976) Summary: 1) An elastolytic enzyme has been isolated from human pancreatic juice. The concentration of the enzyme was about 0.6 mg/m/ corresponding to about 10% of the protein concentration of the juice. 2) The purification procedure included chromatography on Sephadex G-25 followed by ion-exchange chromatography on SP-Sephadex C-50 at pH 6.0, affinity chromatography on TrasylolSepharose 4-B and a final ion-exchange chromatography on SP-Sephadex at pH 7.6. The yield was about 50%. 3) The elastase isolated was homogeneous in analytical disc electrophoresis and showed a single protein component with a molecular weight of 26 300 in dodecylsulfate-electrophoresis. There are similarities in the amino acid compositions of human and porcine pancreatic elastases. 4) The human enzyme has a lower activity on elastin than porcine elastase but similar activities on casein and fibrin. The Km value for Boc-Ala-ONp was 5.13 10~*M. The elastase isolated was 98% active, as judged from active site titration. 5) The results of immunodiffusion studies

Journal

hoppe-seyler's zeitschrift für physiologische chemiede Gruyter

Published: Jan 1, 1976

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off