Introduction Materials and Methods Materials Creatinine, unconjugated bilirubin, glucose, -hydroxybutyric acid, ascorbic acid and acetone were obtained from Merck (Germany), conjugated bilirubin (ditaurate, di Na) from Porphyrin Products Inc. (Logan, Utah, U.S.A.) and human albumin from Behring Werke (Germany). The following drugs were obtained from the Department of Pharmacy in our hospital: 1. cephalosporins -- cephotaxin-Na (Claforan®) - cephalotine-Na (Keflin^) -- cephuroxim-Na (Zinacef®) 2. dopamine^HCl 3. dobutamine-HCl (Dobutrex^) Icteric serum samples were provided by various departments in~ our hospital. Only samples with a total bilirubin concentration higher than 100 / were used. They were stored at -80 °C prior to analysis. Table 1 shows the frequency distribution of the bilirubin concentrations. Cyclosporin-containing serum samples were obtained from our transplantation unit. All cyclosporin concentrations, as determined by radioimmunoassay, were lower than 100 mg/1 which is considered to be therapeutic. Methods An HPLC-based method already described by us (2) was used as the reference method. In a previous study we discussed the problems related to the determination of creatinine in icteric specimens (1). That study was undertaken because of doubt concerning some of our Chem-1 results. We also mentioned the involvement of the Bayer^Technicon company, which manufactures the Chem-1 analyser.
Clinical Chemistry and Laboratory Medicine – de Gruyter
Published: Jan 1, 1993
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