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(1992)
DNA Replication (New York, USA: W.H
M. Sumper, R. Luce (1975)
Evidence for de novo production of self-replicating and environmentally adapted RNA structures by bacteriophage Qbeta replicase.Proceedings of the National Academy of Sciences of the United States of America, 72 1
J. Aliotta, J. Pelletier, J. Ware, L. Moran, J. Benner, H. Kong (1996)
Thermostable Bst DNA polymerase I lacks a 3'-->5' proofreading exonuclease activity.Genetic analysis : biomolecular engineering, 12 5-6
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
C. Biebricher, M. Eigen, R. Luce (1986)
Template-free RNA synthesis by Qβ replicaseNature, 321
E. Rogulin, T. Perevyazova, L. Zheleznaya, N. Matvienko (2004)
Plasmid pRARE as a Vector for Cloning to Construct a Superproducer of the Site-Specific Nickase N.BspD6IBiochemistry (Moscow), 69
Kristijan Ramadan, I. Shevelev, G. Maga, U. Hübscher (2004)
De novo DNA synthesis by human DNA polymerase lambda, DNA polymerase mu and terminal deoxyribonucleotidyl transferase.Journal of molecular biology, 339 2
H. Schachman, J. Adler, C. Radding, I. Lehman, A. Kornberg (1960)
Enzymatic synthesis of deoxyribonucleic acid. VII. Synthesis of a polymer of deoxyadenylate and deoxythymidylate.The Journal of biological chemistry, 235
N. Ogata, T. Miura (1998)
Creation of genetic information by DNA polymerase of the archaeon Thermococcus litoralis: influences of temperature and ionic strength.Nucleic acids research, 26 20
J. King, Cecilia Fairley, W. Morgan (1996)
DNA End Joining by the Klenow Fragment of DNA Polymerase I*The Journal of Biological Chemistry, 271
C. Biebricher, M. Eigen, R. Luce (1981)
Kinetic analysis of template-instructed and de novo RNA synthesis by Q beta replicase.Journal of molecular biology, 148 4
James Clark (1991)
DNA synthesis on discontinuous templates by DNA polymerase I of Escherichia coli.Gene, 104 1
N. Ogata, T. Miura (1997)
Genetic information 'created' by archaebacterial DNA polymerase.The Biochemical journal, 324 ( Pt 2)
C. Brack, J. Griffith (1981)
DNA electron microscopy.CRC critical reviews in biochemistry, 10 2
S. Phang, Chai-Yaw Teo, Evelyn Lo, Victor Wong (1995)
Cloning and complete sequence of the DNA polymerase-encoding gene (BstpolI) and characterisation of the Klenow-like fragment from Bacillus stearothermophilus.Gene, 163 1
N. Ogata, T. Miura (1998)
Creation of genetic information by DNA polymerase of the thermophilic bacterium Thermus thermophilus.Nucleic acids research, 26 20
M. Cho, Linda Wang, J. Choo, Eun Lee (2008)
Loop Mediated Isothermal Amplification of DNA
M. Riggs, Starla Tudor, Mathoor Sivaram, S. Mcdonough (1996)
Construction of single amino acid substitution mutants of cloned Bacillus stearothermophilus DNA polymerase I which lack 5'-->3' exonuclease activity.Biochimica et biophysica acta, 1307 2
C. Brack, J. Griffith (1981)
DNA electron microscopy - part iCritical Reviews in Biochemistry and Molecular Biology, 10
(1987)
Preparation of nucleic acids for electron microscopy
H. Sambrook (1989)
Molecular cloning : a laboratory manual. Cold Spring Harbor, NY
Xingguo Liang, Kari Jensen, M. Frank-Kamenetskii (2004)
Very efficient template/primer-independent DNA synthesis by thermophilic DNA polymerase in the presence of a thermophilic restriction endonuclease.Biochemistry, 43 42
N. Ogata, T. Miura (2000)
Elongation of tandem repetitive DNA by the DNA polymerase of the hyperthermophilic archaeon Thermococcus litoralis at a hairpin-coil transitional state: a model of amplification of a primordial simple DNA sequence.Biochemistry, 39 45
N. Ogata, H. Morino (2000)
Elongation of repetitive DNA by DNA polymerase from a hyperthermophilic bacterium Thermus thermophilus.Nucleic acids research, 28 20
Abstract Highly efficient DNA synthesis without template and primer DNAs occurs when N.BspD6I DNA nickase is added to a reaction mixture containing deoxynucleoside triphosphates and the large fragment of Bst DNA polymerase. Over a period of 2 h, virtually all the deoxynucleoside triphosphates (dNTPs) become incorporated into DNA. Inactivation of N.BspD6I nickase by heating inhibits DNA synthesis. Optimal N.BspD6I activity is required to achieve high yields of synthesized DNA. Electron microscopy data revealed that the majority of DNA molecules have a branched structure. Cloning and sequencing of the fragments synthesized demonstrated that the DNA product mainly consists of multiple hexanucleotide non-palindromic tandem repeats containing nickase recognition sites. A possible mechanism is discussed that addresses template-independent DNA synthesis stimulated by N.BspD6I nickase.
Biological Chemistry – de Gruyter
Published: Apr 1, 2007
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