Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Microwave assisted adsorption based elution: a benign green process optimized by Box-Behnken modeling yields pure vasicine from Adhatoda vasica

Microwave assisted adsorption based elution: a benign green process optimized by Box-Behnken... Abstract Development of economical and rapid high yielding industrial processes for isolation of commercially important plant molecules have been in current industrial vogue. Adhatoda vasica , a functional dietary supplemental plant, is found to harbor vasicine, a commercially important and physiologically active alkaloid. Attempts were made to increase the yields of vasicine by a viable “green” process – microwave assisted adsorption based elution (MAABE) with silica gel. Microwave (300 W) assisted extraction was done with 6% HCl/water (solid loading – 10%), which in 4 h yielded vasicine (8.8 mg/g of leaves, 0.88%). A Box-Behnken designed response surface methodology (RSM) optimized the extraction parameters to 9.31% solid loading, 6.64% HCl/water and 4.1 h, respectively. Further, adsorption based elution using silica gel and desorption with 70% ethanol/water yielded pure vasicine (1.12%) (recovery=93%, purity=95%). The adsorption isotherms studied to understand the adsorption mechanism suggested that the process followed the Langmuir theory. MAABE required less time and labor, in addition to its relative safety and as a “green” alternative process, to give high yields of commercially important vasicine. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Green Processing and Synthesis de Gruyter

Microwave assisted adsorption based elution: a benign green process optimized by Box-Behnken modeling yields pure vasicine from Adhatoda vasica

Loading next page...
 
/lp/de-gruyter/microwave-assisted-adsorption-based-elution-a-benign-green-process-a6IBA1O9N0
Publisher
de Gruyter
Copyright
Copyright © 2016 by the
ISSN
2191-9542
eISSN
2191-9550
DOI
10.1515/gps-2015-0134
Publisher site
See Article on Publisher Site

Abstract

Abstract Development of economical and rapid high yielding industrial processes for isolation of commercially important plant molecules have been in current industrial vogue. Adhatoda vasica , a functional dietary supplemental plant, is found to harbor vasicine, a commercially important and physiologically active alkaloid. Attempts were made to increase the yields of vasicine by a viable “green” process – microwave assisted adsorption based elution (MAABE) with silica gel. Microwave (300 W) assisted extraction was done with 6% HCl/water (solid loading – 10%), which in 4 h yielded vasicine (8.8 mg/g of leaves, 0.88%). A Box-Behnken designed response surface methodology (RSM) optimized the extraction parameters to 9.31% solid loading, 6.64% HCl/water and 4.1 h, respectively. Further, adsorption based elution using silica gel and desorption with 70% ethanol/water yielded pure vasicine (1.12%) (recovery=93%, purity=95%). The adsorption isotherms studied to understand the adsorption mechanism suggested that the process followed the Langmuir theory. MAABE required less time and labor, in addition to its relative safety and as a “green” alternative process, to give high yields of commercially important vasicine.

Journal

Green Processing and Synthesisde Gruyter

Published: Jun 1, 2016

References