Bial. Chem. Hoppe-Seyler Vol. 374, pp. 363-376, June 1993 Microbial Metabolism of Quinoline and Related Compounds XVIII. Purification and Some Properties of the Molybdenum- and Iron-Containing Quinaldic acid 4-oxidoreductase from Serratia marcescens 2CC-1 Susanne FETZNER and Franz LINGENS Institut für Mikrobiologie der Universität Hohenheim, Stuttgart, Germany (Received 27 April 1993) Summary: Serratia marcescens 2CC-1 utilizes quinaldic acid (quinoline 2-carboxylic acid) as sole source of carbon, nitrogen and energy. Growth of strain 2CC-1 on quinaldic acid as well as on nicotinic acid and hypoxanthine was inhibited completely by the molybdate antagonist tungstate, whereas growth on kynurenic acid and 6-hydroxynicotinic acid was not affected by tungstate. The synthesis of the molybdenum-containing hydroxylases quinaldic acid 4-oxidoreductase and nicotinic acid 6-oxidoreductase was found to be inducible. In addition, Serratia marcescens 2CC-1 produced a constitutively expressed xanthine oxidoreductase. Quinaldic acid 4-oxidoreductase was purified 1075fold with a recovery of 5%. For catalytic activity, artificial electron acceptors were necessary. The 95-100kDa enzyme was a heterodimer with subunit molecular masses of 75-80 kDa and 18-19 kDa. Quinaldic acid 4-oxidoreductase contained 2.3-3.7 g atom of iron and 0.5-0.6 g atom of molybdenum per mol of enzyme. The absorption spectrum exhibited maxima at 280 nm, 334 nm, 480
Biological Chemistry Hoppe-Seyler – de Gruyter
Published: Jan 1, 1993
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