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Metabolic Interconversion of Acid Metabolites of Cotinine In Vitro

Metabolic Interconversion of Acid Metabolites of Cotinine In Vitro METABOLIC INTERCONVERSION OF ACID METABOLITES OF COTININE /TV VITRO Yang Sai and John W. Gorrod* Chelsea Department of Pharmacy, King's College London, University of London, Manresa Road, London SIV3 6LX, U.K. SUMMARY The in vitro metabolism of 4-(3-pyridyl)-4-oxo-butyric acid (POBA), 4-(3-pyridyl)-4-hydroxy-butyric acid (PHBA) and 5-(3pyridyl)-tetrahydrofiiranone-2 (PTHF) by hepatic fractions from different experimental animal species (rat, rabbit, hamster, guinea-pig) was studied. The results show that POBA can be reduced to PHBA followed by cyclisation to PTHF by enzymes present in the cell soluble fraction. Conversely PHBA can be oxidised to POBA in vitro by microsomal enzymes. The metabolic transformations observed are predominantly catalysed by enzymes. PTHF is transformed to PHBA by a chemical hydrolytic reaction which is enhanced by both microsomal and soluble enzymes. Under our HPLC analytical conditions, neither 3-pyridylacetic acid nor 4-(3-pyridyl)-butyric acid were detected. Among the four species studied, there was a significant difference in the reduction of POBA to PHBA, with rabbit and hamster having the stronger activity. When the three compounds were incubated with hepatic tissue from rats induced separately with phenobarbitone (PB), ß-naphthoflavone (ßNF) or arochlor-1254 (ΑΙ 254), no difference in interconversion of the metabolites was observed when compared to non-induced rats. Therefore, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Drug Metabolism and Drug Interactions de Gruyter

Metabolic Interconversion of Acid Metabolites of Cotinine In Vitro

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References (4)

Publisher
de Gruyter
Copyright
Copyright © 1995 by the
ISSN
2191-0162
eISSN
2191-0162
DOI
10.1515/DMDI.1995.12.2.93
Publisher site
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Abstract

METABOLIC INTERCONVERSION OF ACID METABOLITES OF COTININE /TV VITRO Yang Sai and John W. Gorrod* Chelsea Department of Pharmacy, King's College London, University of London, Manresa Road, London SIV3 6LX, U.K. SUMMARY The in vitro metabolism of 4-(3-pyridyl)-4-oxo-butyric acid (POBA), 4-(3-pyridyl)-4-hydroxy-butyric acid (PHBA) and 5-(3pyridyl)-tetrahydrofiiranone-2 (PTHF) by hepatic fractions from different experimental animal species (rat, rabbit, hamster, guinea-pig) was studied. The results show that POBA can be reduced to PHBA followed by cyclisation to PTHF by enzymes present in the cell soluble fraction. Conversely PHBA can be oxidised to POBA in vitro by microsomal enzymes. The metabolic transformations observed are predominantly catalysed by enzymes. PTHF is transformed to PHBA by a chemical hydrolytic reaction which is enhanced by both microsomal and soluble enzymes. Under our HPLC analytical conditions, neither 3-pyridylacetic acid nor 4-(3-pyridyl)-butyric acid were detected. Among the four species studied, there was a significant difference in the reduction of POBA to PHBA, with rabbit and hamster having the stronger activity. When the three compounds were incubated with hepatic tissue from rats induced separately with phenobarbitone (PB), ß-naphthoflavone (ßNF) or arochlor-1254 (ΑΙ 254), no difference in interconversion of the metabolites was observed when compared to non-induced rats. Therefore,

Journal

Drug Metabolism and Drug Interactions – de Gruyter

Published: Jun 1, 1995

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