Lipid-Lipid and Lipid-Protein Interactions as Studied with a Novel Type of Fluorescent Fatty Acid and Phospholipid Probes

Lipid-Lipid and Lipid-Protein Interactions as Studied with a Novel Type of Fluorescent Fatty Acid... Hoppe-Seyler'sZ. Physiol. Chem. Bd. 357, S. 21 - 33, Januar 1976 Wilhelm STOFFEL and Gabriele MICHAELIS Institut für Physiologische Chemie der Universität zu Köln (Received 3 November 1975) Summary: A novel fluorescent-labelled group of fatty acids and phospholipids has been applied to determine phase transitions in liposomes by fluorescence intensity and polarisation measurements. The chromophore of these amphiphilic lipids proved to be very suitable to demonstrate temperature-dependent lipid-lipid interactions. Liposomes from l,2-dipalmitoyl-3-5«-glycerophosphocholine, l-stearoyl-2-oleoyl-3-s«-grycero- phosphoethanolamine and from lipids isolated from membranes of E. coli K 1062 mutant grown on elaidic acid were used in these studies. These probes also made it possible to observe conformational changes in membrane proteins in isolated plasma membranes from this mutant. The changes in protein conformation were dependent on structural changes in the lipid phase. Untersuchung von Lipid-Lipid- und Lipid-Protein-Wechselwirkungen mit Hilfe neuer fluoreszenzmarkierter Fettsäuren und Phospholipide Zusammenfassung: Mit Hilfe neuer fluoreszenzmarkierter Fettsäuren und Phospholipide wurden Phasenumwandlungen an Liposomen durch Fluoreszenzintensitäts- und Fluoreszenzpolarisationsmessungen bestimmt. Der Chromophor in diesen amphiphilen Lipiden erwies sich als sehr geeignet, temperaturabhängige Lipid-Lipid-Wechselwirkungen anzuzeigen. Sie wurden in Liposomen aus l ,2-Dipalmitoyl-3-s«-glycerophosphocholin, aus l -Stearoyl-2-oleoyl-3-s«-giycerophosphoäthanolamin sowie an solchen, die aus Membranlipiden der auf Elaidinsäure gewachsenen E. coli Bakterienmutante K 1062 hergestellt http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png hoppe-seyler's zeitschrift für physiologische chemie de Gruyter

Lipid-Lipid and Lipid-Protein Interactions as Studied with a Novel Type of Fluorescent Fatty Acid and Phospholipid Probes

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Publisher
de Gruyter
Copyright
Copyright © 1976 by the
ISSN
0018-4888
eISSN
1437-4315
DOI
10.1515/bchm2.1976.357.1.21
Publisher site
See Article on Publisher Site

Abstract

Hoppe-Seyler'sZ. Physiol. Chem. Bd. 357, S. 21 - 33, Januar 1976 Wilhelm STOFFEL and Gabriele MICHAELIS Institut für Physiologische Chemie der Universität zu Köln (Received 3 November 1975) Summary: A novel fluorescent-labelled group of fatty acids and phospholipids has been applied to determine phase transitions in liposomes by fluorescence intensity and polarisation measurements. The chromophore of these amphiphilic lipids proved to be very suitable to demonstrate temperature-dependent lipid-lipid interactions. Liposomes from l,2-dipalmitoyl-3-5«-glycerophosphocholine, l-stearoyl-2-oleoyl-3-s«-grycero- phosphoethanolamine and from lipids isolated from membranes of E. coli K 1062 mutant grown on elaidic acid were used in these studies. These probes also made it possible to observe conformational changes in membrane proteins in isolated plasma membranes from this mutant. The changes in protein conformation were dependent on structural changes in the lipid phase. Untersuchung von Lipid-Lipid- und Lipid-Protein-Wechselwirkungen mit Hilfe neuer fluoreszenzmarkierter Fettsäuren und Phospholipide Zusammenfassung: Mit Hilfe neuer fluoreszenzmarkierter Fettsäuren und Phospholipide wurden Phasenumwandlungen an Liposomen durch Fluoreszenzintensitäts- und Fluoreszenzpolarisationsmessungen bestimmt. Der Chromophor in diesen amphiphilen Lipiden erwies sich als sehr geeignet, temperaturabhängige Lipid-Lipid-Wechselwirkungen anzuzeigen. Sie wurden in Liposomen aus l ,2-Dipalmitoyl-3-s«-glycerophosphocholin, aus l -Stearoyl-2-oleoyl-3-s«-giycerophosphoäthanolamin sowie an solchen, die aus Membranlipiden der auf Elaidinsäure gewachsenen E. coli Bakterienmutante K 1062 hergestellt

Journal

hoppe-seyler's zeitschrift für physiologische chemiede Gruyter

Published: Jan 1, 1976

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