Investigation of Total and Conjugated Bilirubin Determination during the Neonatal Period

Investigation of Total and Conjugated Bilirubin Determination during the Neonatal Period Introduction Neonatal hyperbilirubinaemia is currently observed during the neonatal period (25 -r 50% of all neonates), mostly due to low activity of the hepatic glucuronosyltransferase (EC 2.4.1.17) (1). Total and conjugated (or "direct") bilirubin determinations are necessary to identify the origin of the jaundice, to predict its evolution and to treat it. Visible light phototherapy is one of the effective treatments frequently given to these children, but it produces photochemical prod- ucts (2) that may interfere with the biochemical methods commonly used for bilirubin determination (3). The aim of this study is to compare the data of total and conjugated bilirubin measured in neonates, undergoing phototherapy or not, using two commonly employed methods: colorimetric diazo reaction on a Hitachi 717, dual wavelength reflectance on a Kodak Ektachem 700. High performance liquid chromatograpfay was then used for further investigation. ,_ , Eur. J. Clin. Chera. din. Biochem. / Vol. 31,1993 / No. 8 Materials and Methods Patient samples Serum samples were from 108 neonates during the first 15 days of life (mean age: 4.7 days; range: 1-14 days), submitted to the laboratory for a survey of the bilirubin course. The gestational ages of the infants ranged from 26 to http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Investigation of Total and Conjugated Bilirubin Determination during the Neonatal Period

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1993.31.8.499
Publisher site
See Article on Publisher Site

Abstract

Introduction Neonatal hyperbilirubinaemia is currently observed during the neonatal period (25 -r 50% of all neonates), mostly due to low activity of the hepatic glucuronosyltransferase (EC 2.4.1.17) (1). Total and conjugated (or "direct") bilirubin determinations are necessary to identify the origin of the jaundice, to predict its evolution and to treat it. Visible light phototherapy is one of the effective treatments frequently given to these children, but it produces photochemical prod- ucts (2) that may interfere with the biochemical methods commonly used for bilirubin determination (3). The aim of this study is to compare the data of total and conjugated bilirubin measured in neonates, undergoing phototherapy or not, using two commonly employed methods: colorimetric diazo reaction on a Hitachi 717, dual wavelength reflectance on a Kodak Ektachem 700. High performance liquid chromatograpfay was then used for further investigation. ,_ , Eur. J. Clin. Chera. din. Biochem. / Vol. 31,1993 / No. 8 Materials and Methods Patient samples Serum samples were from 108 neonates during the first 15 days of life (mean age: 4.7 days; range: 1-14 days), submitted to the laboratory for a survey of the bilirubin course. The gestational ages of the infants ranged from 26 to

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1993

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