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Interaction of Non-Steroidal Anti-Inflammatory Drugs, Etoricoxib and Parecoxib Sodium, with Human Serum Albumin Studied by Fluorescence Spectroscopy

Interaction of Non-Steroidal Anti-Inflammatory Drugs, Etoricoxib and Parecoxib Sodium, with Human... Neelam Seedher* and Sonu Bhatia Department of Chemistry, Panjab University, Chandigarh, India SUMMARY The mechanism of interaction of the non-steroidal anti-inflammatory drugs, etoricoxib and parecoxib sodium, with human serum albumin (HSA) was studied using fluorescence spectroscopy. There was only one class of binding site with association constants of the order of 104. Thermodynamic parameters suggest that van der Waals and hydrogen bonding in the case of etoricoxib, and electrostatic and hydrogen bonding in the case of parecoxib sodium, are predominantly involved in the binding. Studies in the presence of the hydrophobic probe, 1-anilinonaphthalene-8sulfonate (ANS), showed that hydrophobic are not involved in the binding of these drugs to HSA. Displacement studies using the site-specific probe, dansylsarcosine piperidinium salt (DSS), showed that the drugs are bound at site II on the HSA molecule. However, etoricoxib and parecoxib sodium are bound at different regions within site II. Increase of pH and the presence of salt caused significant changes in the association constants and the concentration of free pharmacologically active drug. Stern-Volmer analysis of the binding data indicated that the tryptophan residues of albumin are not * Author for correspondence: Neelam Seedher Department of Chemistry Panjab University Chandigarh, India e-mail: nseedher@yahoo.com ©Freund http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Drug Metabolism and Drug Interactions de Gruyter

Interaction of Non-Steroidal Anti-Inflammatory Drugs, Etoricoxib and Parecoxib Sodium, with Human Serum Albumin Studied by Fluorescence Spectroscopy

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References (47)

Publisher
de Gruyter
Copyright
Copyright © 2006 by the
ISSN
2191-0162
eISSN
2191-0162
DOI
10.1515/DMDI.2006.22.1.25
Publisher site
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Abstract

Neelam Seedher* and Sonu Bhatia Department of Chemistry, Panjab University, Chandigarh, India SUMMARY The mechanism of interaction of the non-steroidal anti-inflammatory drugs, etoricoxib and parecoxib sodium, with human serum albumin (HSA) was studied using fluorescence spectroscopy. There was only one class of binding site with association constants of the order of 104. Thermodynamic parameters suggest that van der Waals and hydrogen bonding in the case of etoricoxib, and electrostatic and hydrogen bonding in the case of parecoxib sodium, are predominantly involved in the binding. Studies in the presence of the hydrophobic probe, 1-anilinonaphthalene-8sulfonate (ANS), showed that hydrophobic are not involved in the binding of these drugs to HSA. Displacement studies using the site-specific probe, dansylsarcosine piperidinium salt (DSS), showed that the drugs are bound at site II on the HSA molecule. However, etoricoxib and parecoxib sodium are bound at different regions within site II. Increase of pH and the presence of salt caused significant changes in the association constants and the concentration of free pharmacologically active drug. Stern-Volmer analysis of the binding data indicated that the tryptophan residues of albumin are not * Author for correspondence: Neelam Seedher Department of Chemistry Panjab University Chandigarh, India e-mail: nseedher@yahoo.com ©Freund

Journal

Drug Metabolism and Drug Interactions – de Gruyter

Published: Dec 1, 2006

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