Influence of Insulin on Lysosomal Activity and Urea Production in Isolated Parenchymal Cells from Rat Liver

Influence of Insulin on Lysosomal Activity and Urea Production in Isolated Parenchymal Cells from... Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 977 - 981, Juli 1976 ReinertGRAMMELTVEDT and Trond BERG Institute of Zoophysiology, University of Oslo, Blindem (Received 16 February 1976) Summary: Non-latent (free) activities of two lysosomal enzymes (acid phosphatase and ß-glucuronidase) and urea production were measured in purified rat liver parenchymal cells incubated in the presence and absence of insulin. Non-latent enzyme activity was measured by including 0.25M sucrose in the assay mixtures to provide osmotic protection to the lysosomes. Total en- zyme activity was estimated with Triton X-100 in the homogenates. Insulin was found to inhibit ureogenesis and to reduce non-latent lysosomal enzyme activity in the hepatocytes in vitro. Our data support the idea that insulin inhibits autophagy in rat liver parenchymal cells. Such an effect of insulin may also explain the inhibitory action of insulin on urea production in the rat liver. Einfluß von Insulin auf die Aktivität der Lysosomen und die Harnstoff-Bildung in isolierten Rattenleber-Parenchymzellen Zusammenfassung: Die freien Aktivitäten lysosomaler Enzyme (der sauren Phosphatase und der ß-Glucuronidase) sowie die Harnstoff-Bildung wurden in gereinigten Rattenleber-Parenchymzellen nach Inkubation in Gegenwart und Abwesenheit von Insulin gemessen. Zur Vermeidung der Zellyse wurde bei der Messung 0.25M Saccharose zugesetzt. Die Gesamtenzymaktivität in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png hoppe-seyler's zeitschrift für physiologische chemie de Gruyter

Influence of Insulin on Lysosomal Activity and Urea Production in Isolated Parenchymal Cells from Rat Liver

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Publisher
de Gruyter
Copyright
Copyright © 1976 by the
ISSN
0018-4888
eISSN
1437-4315
DOI
10.1515/bchm2.1976.357.2.977
Publisher site
See Article on Publisher Site

Abstract

Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 977 - 981, Juli 1976 ReinertGRAMMELTVEDT and Trond BERG Institute of Zoophysiology, University of Oslo, Blindem (Received 16 February 1976) Summary: Non-latent (free) activities of two lysosomal enzymes (acid phosphatase and ß-glucuronidase) and urea production were measured in purified rat liver parenchymal cells incubated in the presence and absence of insulin. Non-latent enzyme activity was measured by including 0.25M sucrose in the assay mixtures to provide osmotic protection to the lysosomes. Total en- zyme activity was estimated with Triton X-100 in the homogenates. Insulin was found to inhibit ureogenesis and to reduce non-latent lysosomal enzyme activity in the hepatocytes in vitro. Our data support the idea that insulin inhibits autophagy in rat liver parenchymal cells. Such an effect of insulin may also explain the inhibitory action of insulin on urea production in the rat liver. Einfluß von Insulin auf die Aktivität der Lysosomen und die Harnstoff-Bildung in isolierten Rattenleber-Parenchymzellen Zusammenfassung: Die freien Aktivitäten lysosomaler Enzyme (der sauren Phosphatase und der ß-Glucuronidase) sowie die Harnstoff-Bildung wurden in gereinigten Rattenleber-Parenchymzellen nach Inkubation in Gegenwart und Abwesenheit von Insulin gemessen. Zur Vermeidung der Zellyse wurde bei der Messung 0.25M Saccharose zugesetzt. Die Gesamtenzymaktivität in

Journal

hoppe-seyler's zeitschrift für physiologische chemiede Gruyter

Published: Jan 1, 1976

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