Influence of Auxiliary Enzymes on the Spectrophotometric Measurement of Alanine Aminotransferase and Aspartate Aminotransferase Activities

Influence of Auxiliary Enzymes on the Spectrophotometric Measurement of Alanine Aminotransferase... Jung, Fechner and Egger: Influence of auxiliary enzymes on the measurement of aminotransferase activities J. Clin. Chem. Clin. Biochem. VoL14, 1976, pp. 53-57 By K. Jungj Christa Fechner and E. Egger Department of Clinical Biochemistryf Charite, Humboldt University Berlin (Eingegangen am 11. Juli/30. September 1975) Summary: We investigated the enzyme activity of the blank in the Spectrophotometric determination of alanine aminotransferase activities and aspartate aminotransferase activity. 6 lactate dehydrogenase and 3 malate dehydrogenase preparations from different manufacturers and from different organs showed additional and contaminating activity. The additional activity depends upon the 2-oxoglutarate concentration. The contaminating activity is caused by alanine aminotransferase and aspartate aminotransferase in the auxiliary enzymes. We propose that exact definitions must be given for the auxiliary enzymes in the recommendations of standard determinations for enzyme activities. Einfluß der Hilfsenzyme auf die spektrophotometrische Bestimmung der Aktivität von Alanin- undAspartataminotransferase Zusammenfassung: 6 Lactatdehydrogenase- und 3 Malatdehydrogenase-Präparate verschiedener Hersteller und unterschiedlicher Organherkunft wurden hinsichtlich der Leerwert-Aktivität bei der Alanin- bzw. Aspartataminotransferase-Aktivitätsbestimmung mit dem UV-Test untersucht. Sie zeigten unterschiedlich hohe Neben- und Fremdaktivitätsanteile. Die Nebenaktivität war von der 2-Oxoghitarat-Konzentration abhängig. Die Fremdaktivität war in erster Linie durch die in den Hilfsenzymen enthaltene Alanin- und Aspartataminotransferase bedingt. Es wird http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Influence of Auxiliary Enzymes on the Spectrophotometric Measurement of Alanine Aminotransferase and Aspartate Aminotransferase Activities

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1976.14.1-12.53
Publisher site
See Article on Publisher Site

Abstract

Jung, Fechner and Egger: Influence of auxiliary enzymes on the measurement of aminotransferase activities J. Clin. Chem. Clin. Biochem. VoL14, 1976, pp. 53-57 By K. Jungj Christa Fechner and E. Egger Department of Clinical Biochemistryf Charite, Humboldt University Berlin (Eingegangen am 11. Juli/30. September 1975) Summary: We investigated the enzyme activity of the blank in the Spectrophotometric determination of alanine aminotransferase activities and aspartate aminotransferase activity. 6 lactate dehydrogenase and 3 malate dehydrogenase preparations from different manufacturers and from different organs showed additional and contaminating activity. The additional activity depends upon the 2-oxoglutarate concentration. The contaminating activity is caused by alanine aminotransferase and aspartate aminotransferase in the auxiliary enzymes. We propose that exact definitions must be given for the auxiliary enzymes in the recommendations of standard determinations for enzyme activities. Einfluß der Hilfsenzyme auf die spektrophotometrische Bestimmung der Aktivität von Alanin- undAspartataminotransferase Zusammenfassung: 6 Lactatdehydrogenase- und 3 Malatdehydrogenase-Präparate verschiedener Hersteller und unterschiedlicher Organherkunft wurden hinsichtlich der Leerwert-Aktivität bei der Alanin- bzw. Aspartataminotransferase-Aktivitätsbestimmung mit dem UV-Test untersucht. Sie zeigten unterschiedlich hohe Neben- und Fremdaktivitätsanteile. Die Nebenaktivität war von der 2-Oxoghitarat-Konzentration abhängig. Die Fremdaktivität war in erster Linie durch die in den Hilfsenzymen enthaltene Alanin- und Aspartataminotransferase bedingt. Es wird

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1976

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