Identification and Partial Characterization of Specific Oestrogen-Binding Components in Human Kidney

Identification and Partial Characterization of Specific Oestrogen-Binding Components in Human Kidney Introduction Although the kidney is not generally regarded as one of the oestrogen target organs, the induction of hamster kidney tumors by prolonged oestrogen administration is well-known (1, 2). One of the reasons for the paucity of information on the oestrogen responsiveness of kidney is the former paradoxical failure to demonstrate oestrogen receptors in normal hamster kidney (3). However, there are some indications of oestradiol J. Clin. Chem. Clin. Biochem. / Vol. 14, 1976 / No. 1 binding to cytosol macromolecules and the nuclear fraction of rat kidney (4, 5). Moreover, oestrogens have been shown to decrease renal sodium excretion in adrenalectomized rats (4), dogs (6), and normal humans (7). These studies prompted an examination of normal human kidney for oestrogen-binding components. A partial characterisation of these binding sites is presented in this paper. Portions of these data have been reported in a preliminary form (8). Materials and Methods Bojar, Balzer, Dreyf rst, Staib and Wittliff: Identification of oestrogen receptor in human kidney SW56 titanium rotor. After centrifugation the bottom of the tubes were pierced and six-drop fractions collected into scintillation vials containing 2 ml of 99% ethanol. Ten milliliters of toluene scintillation cocktail (4 g Omriifluor/1 toluene) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Identification and Partial Characterization of Specific Oestrogen-Binding Components in Human Kidney

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1976.14.1-12.515
Publisher site
See Article on Publisher Site

Abstract

Introduction Although the kidney is not generally regarded as one of the oestrogen target organs, the induction of hamster kidney tumors by prolonged oestrogen administration is well-known (1, 2). One of the reasons for the paucity of information on the oestrogen responsiveness of kidney is the former paradoxical failure to demonstrate oestrogen receptors in normal hamster kidney (3). However, there are some indications of oestradiol J. Clin. Chem. Clin. Biochem. / Vol. 14, 1976 / No. 1 binding to cytosol macromolecules and the nuclear fraction of rat kidney (4, 5). Moreover, oestrogens have been shown to decrease renal sodium excretion in adrenalectomized rats (4), dogs (6), and normal humans (7). These studies prompted an examination of normal human kidney for oestrogen-binding components. A partial characterisation of these binding sites is presented in this paper. Portions of these data have been reported in a preliminary form (8). Materials and Methods Bojar, Balzer, Dreyf rst, Staib and Wittliff: Identification of oestrogen receptor in human kidney SW56 titanium rotor. After centrifugation the bottom of the tubes were pierced and six-drop fractions collected into scintillation vials containing 2 ml of 99% ethanol. Ten milliliters of toluene scintillation cocktail (4 g Omriifluor/1 toluene)

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1976

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