Hydrolysis-Resynthesis Equilibrium of the Lysine-15–Alanine-16 Peptide Bond in Bovine Trypsin Inhibitor (Kunitz)

Hydrolysis-Resynthesis Equilibrium of the Lysine-15–Alanine-16 Peptide Bond in Bovine Trypsin... Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 769 - 776, Juni 1976 Hydrolysis-Resy n thesis Equilibrium of the Lysine-15--Alanine-16 Peptide Bond in Bovine Trypsin Inhibitor (Kunitz) Harald TSCHESCHE and Sigrid KUPFER Organisch-Chemisches Institut der Technischen Universität München Lehrstuhl für Organische Chemie und Biochemie (Received 13 April 1976) Dedicated to Prof. H. Zahn on the occasion of his 60th birthday Summary: Catalytic amounts of bovine ß-trypsin, equilibrium concentrations of virgin and modified bovine -chymotrypsin and porcine plasmin inhibitor are established by plasmin after almost establish a true thermodynamic equilibrium 300 days starting from either pure virgin or pure 15 16 between virgin (I) (reactive site Lys -Ala modified inhibitor. Thus, the hydrolysis constant peptide bond intact) and modified (I*) (this bond ^Hyd = [!*]/[!] is determined to be 0.33 at pH 5.O. In spite of many unsuccessful attempts, hydrolyzed) bovine trypsin/kallikrein inhibitor this demonstrates that the reactive site peptide (Kunitz). The very slow reaction rates for attainbond Lys15-Ala16 in the bovine trypsin inhibitor ing equilibrium are pH-dependent and differ for different enzymes. Optimal rates are for ß-trypsin (Kunitz) can be hydrolyzed by catalytic amounts at pH 3.75, for -chymotrypsin at pH 5.5, and for of endopeptidase. It further confirms http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png hoppe-seyler's zeitschrift für physiologische chemie de Gruyter

Hydrolysis-Resynthesis Equilibrium of the Lysine-15–Alanine-16 Peptide Bond in Bovine Trypsin Inhibitor (Kunitz)

Loading next page...
 
/lp/de-gruyter/hydrolysis-resynthesis-equilibrium-of-the-lysine-15-alanine-16-peptide-0KFQXwSON2
Publisher
de Gruyter
Copyright
Copyright © 1976 by the
ISSN
0018-4888
eISSN
1437-4315
DOI
10.1515/bchm2.1976.357.1.769
Publisher site
See Article on Publisher Site

Abstract

Hoppe-Seyler's Z. Physiol. Chem. Bd. 357, S. 769 - 776, Juni 1976 Hydrolysis-Resy n thesis Equilibrium of the Lysine-15--Alanine-16 Peptide Bond in Bovine Trypsin Inhibitor (Kunitz) Harald TSCHESCHE and Sigrid KUPFER Organisch-Chemisches Institut der Technischen Universität München Lehrstuhl für Organische Chemie und Biochemie (Received 13 April 1976) Dedicated to Prof. H. Zahn on the occasion of his 60th birthday Summary: Catalytic amounts of bovine ß-trypsin, equilibrium concentrations of virgin and modified bovine -chymotrypsin and porcine plasmin inhibitor are established by plasmin after almost establish a true thermodynamic equilibrium 300 days starting from either pure virgin or pure 15 16 between virgin (I) (reactive site Lys -Ala modified inhibitor. Thus, the hydrolysis constant peptide bond intact) and modified (I*) (this bond ^Hyd = [!*]/[!] is determined to be 0.33 at pH 5.O. In spite of many unsuccessful attempts, hydrolyzed) bovine trypsin/kallikrein inhibitor this demonstrates that the reactive site peptide (Kunitz). The very slow reaction rates for attainbond Lys15-Ala16 in the bovine trypsin inhibitor ing equilibrium are pH-dependent and differ for different enzymes. Optimal rates are for ß-trypsin (Kunitz) can be hydrolyzed by catalytic amounts at pH 3.75, for -chymotrypsin at pH 5.5, and for of endopeptidase. It further confirms

Journal

hoppe-seyler's zeitschrift für physiologische chemiede Gruyter

Published: Jan 1, 1976

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off