Human Pharmacokinetics of Orally Administered (24 R)-Hydroxycalcidiol

Human Pharmacokinetics of Orally Administered (24 R)-Hydroxycalcidiol Introduction It is generally accepted that calcitriol (1,251hydroxycholecalciferol) represents the biologically active metabolite of vitamin D3 (1), while the function of (24A)-hydroxycalcidiol is still controversial. Human pharmacokinetics of (24/?)-hydroxycalcidiol have been studied (2 -- 5), but only one of these studies yielded reliable estimates for its clearance and proEur. J. Ctin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 7 duction rate (5). In vitro and in vivo studies have demonstrated that the activity of rat 24-hydroxylase is modulated by parathyroid hormone (parathyrin) (6-8), calcitriol (7-11), dietary calcium (7, 10-13), and phosphorus (7, 10). Hence, production rate or clearance of (24/?)-hydroxycalcidiol may be determined by these factors and may reflect abnormalities of calcium and vitamin D3 metabolism. Leeuwenkamp et al.: Human pharmacokinetics of (24 A)-hydroxycalcidiol Aliquots of these pooled samples were chromatographed on the Chromatographie system used in our assay procedure (15), the (24-K)-hydroxycalcidiol fractions were collected, the eluent evaporated under a stream of nitrogen and the residue dissolved in 480 of ethanol. Of this solution 350 were used to determine (24A)-hydroxycalcidiol; 100 were rechromatographed on a 30-cm d8 column (Spherisorb ODS 2) with 95 : 5 methanol-water as eluent (1.0 ml/miri), the (24^)-hydroxycalcidiol fractions were collected (recovery http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1993.31.7.419
Publisher site
See Article on Publisher Site

Abstract

Introduction It is generally accepted that calcitriol (1,251hydroxycholecalciferol) represents the biologically active metabolite of vitamin D3 (1), while the function of (24A)-hydroxycalcidiol is still controversial. Human pharmacokinetics of (24/?)-hydroxycalcidiol have been studied (2 -- 5), but only one of these studies yielded reliable estimates for its clearance and proEur. J. Ctin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 7 duction rate (5). In vitro and in vivo studies have demonstrated that the activity of rat 24-hydroxylase is modulated by parathyroid hormone (parathyrin) (6-8), calcitriol (7-11), dietary calcium (7, 10-13), and phosphorus (7, 10). Hence, production rate or clearance of (24/?)-hydroxycalcidiol may be determined by these factors and may reflect abnormalities of calcium and vitamin D3 metabolism. Leeuwenkamp et al.: Human pharmacokinetics of (24 A)-hydroxycalcidiol Aliquots of these pooled samples were chromatographed on the Chromatographie system used in our assay procedure (15), the (24-K)-hydroxycalcidiol fractions were collected, the eluent evaporated under a stream of nitrogen and the residue dissolved in 480 of ethanol. Of this solution 350 were used to determine (24A)-hydroxycalcidiol; 100 were rechromatographed on a 30-cm d8 column (Spherisorb ODS 2) with 95 : 5 methanol-water as eluent (1.0 ml/miri), the (24^)-hydroxycalcidiol fractions were collected (recovery

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1993

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