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Evaluation of a Test Kit for the Rapid and Simple Colorimetric Measurement of Angiotensin I-Converting Enzyme in Serum

Evaluation of a Test Kit for the Rapid and Simple Colorimetric Measurement of Angiotensin... Introduction Determination of the serum catalytic activity of angiotensin I-converting enzyme (EC 3.4.15.1) is increasingly being used äs a vatuable tool in the diagnosis and inanagement of sarcoidosis (1--4) and certain other diseases such äs silicosis (5), asbestosis (5), Gaucher's disease (6) and, possibly, malignant J. Clin. Chem. Clin. Biochem. / Vol. 21,1983 / No. 12 histiocytosis (7, 8). Serum angiotensin I-converting enzyme is elevated in the active stages of these diseases, äs it is in hyperthyroidism (9), in chronic liver disease (10), and in diabetes mellitus with severe retinopathy (l 1). In contrast, serum angiotensin I-converting enzyme is depressed in patients with malignant lymphomas (12), leukaemia (12), multiple myeloma (12) and lung cancer (13). Boomsma and Schalekamp: Evaluation of a colour lest kit for angiotensin I-converting enzyme Then l .5 ml of the stopper/developer solution is added, and after mixing and incubating at 37 °C fof 3 minutes, the absorbance is read at 505 nm against distilled water. Blanks are prepared by adding the sample serum aftef the 20 minutes incubation Step. We have modified this procedure in three ways: 1) By halving the amounts of serum and reagents used and ernploying semimicrocuvettes, twice s many samples http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Evaluation of a Test Kit for the Rapid and Simple Colorimetric Measurement of Angiotensin I-Converting Enzyme in Serum

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1983.21.12.845
Publisher site
See Article on Publisher Site

Abstract

Introduction Determination of the serum catalytic activity of angiotensin I-converting enzyme (EC 3.4.15.1) is increasingly being used äs a vatuable tool in the diagnosis and inanagement of sarcoidosis (1--4) and certain other diseases such äs silicosis (5), asbestosis (5), Gaucher's disease (6) and, possibly, malignant J. Clin. Chem. Clin. Biochem. / Vol. 21,1983 / No. 12 histiocytosis (7, 8). Serum angiotensin I-converting enzyme is elevated in the active stages of these diseases, äs it is in hyperthyroidism (9), in chronic liver disease (10), and in diabetes mellitus with severe retinopathy (l 1). In contrast, serum angiotensin I-converting enzyme is depressed in patients with malignant lymphomas (12), leukaemia (12), multiple myeloma (12) and lung cancer (13). Boomsma and Schalekamp: Evaluation of a colour lest kit for angiotensin I-converting enzyme Then l .5 ml of the stopper/developer solution is added, and after mixing and incubating at 37 °C fof 3 minutes, the absorbance is read at 505 nm against distilled water. Blanks are prepared by adding the sample serum aftef the 20 minutes incubation Step. We have modified this procedure in three ways: 1) By halving the amounts of serum and reagents used and ernploying semimicrocuvettes, twice s many samples

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1983

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