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Introduction Determination of the serum catalytic activity of angiotensin I-converting enzyme (EC 3.4.15.1) is increasingly being used äs a vatuable tool in the diagnosis and inanagement of sarcoidosis (1--4) and certain other diseases such äs silicosis (5), asbestosis (5), Gaucher's disease (6) and, possibly, malignant J. Clin. Chem. Clin. Biochem. / Vol. 21,1983 / No. 12 histiocytosis (7, 8). Serum angiotensin I-converting enzyme is elevated in the active stages of these diseases, äs it is in hyperthyroidism (9), in chronic liver disease (10), and in diabetes mellitus with severe retinopathy (l 1). In contrast, serum angiotensin I-converting enzyme is depressed in patients with malignant lymphomas (12), leukaemia (12), multiple myeloma (12) and lung cancer (13). Boomsma and Schalekamp: Evaluation of a colour lest kit for angiotensin I-converting enzyme Then l .5 ml of the stopper/developer solution is added, and after mixing and incubating at 37 °C fof 3 minutes, the absorbance is read at 505 nm against distilled water. Blanks are prepared by adding the sample serum aftef the 20 minutes incubation Step. We have modified this procedure in three ways: 1) By halving the amounts of serum and reagents used and ernploying semimicrocuvettes, twice s many samples
Clinical Chemistry and Laboratory Medicine – de Gruyter
Published: Jan 1, 1983
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