Introduction Bromcresol green and, to a lesser degree, bromcresol purple are widely used "chromogens" for the rapid colorimetric determination of albumin in serum (1--6). In spite of its lower specificity, bromcresol green has often been preferred over bromcresol purple in commercial reagents (7--9). Arguments against bromcresol purple include: 1) underestimation of albumin in cases of renal insufficiency (10) and obstructive jaundice (11); 2) lesser precision and sensitivity of bromcresol purple assays because of the 2 times lower molar absorptivity for the bromcresol purple-albumin dye complex as compared with bromcresol greenalbumin (9); 3) difficulties in the standardization and monitoring of bromcresol purple-based assays with non-human fluids, due to the fact that bromcresol purple -- unlike bromcresol green -- does not strongly bind to bovine serum albumin, often present in calibrators and quality control materials (9). On the other hand, bromcresol green suffers from a more pronounced reactivity with various globulins (5). These undesirable side-reactions proceed, howEur. J. Clin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 3 ever, at a much slower rate. Consequently, by making spectrophotometric readings shortly after the addition of reagent, the specificity of bromcresol green assays for albumin is safeguarded, keeping it at a level
Clinical Chemistry and Laboratory Medicine – de Gruyter
Published: Jan 1, 1993
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