Ektachem Slides Overestimate Serum Albumin in Patients with Nephrotic Syndrome

Ektachem Slides Overestimate Serum Albumin in Patients with Nephrotic Syndrome Introduction Bromcresol green and, to a lesser degree, bromcresol purple are widely used "chromogens" for the rapid colorimetric determination of albumin in serum (1--6). In spite of its lower specificity, bromcresol green has often been preferred over bromcresol purple in commercial reagents (7--9). Arguments against bromcresol purple include: 1) underestimation of albumin in cases of renal insufficiency (10) and obstructive jaundice (11); 2) lesser precision and sensitivity of bromcresol purple assays because of the 2 times lower molar absorptivity for the bromcresol purple-albumin dye complex as compared with bromcresol greenalbumin (9); 3) difficulties in the standardization and monitoring of bromcresol purple-based assays with non-human fluids, due to the fact that bromcresol purple -- unlike bromcresol green -- does not strongly bind to bovine serum albumin, often present in calibrators and quality control materials (9). On the other hand, bromcresol green suffers from a more pronounced reactivity with various globulins (5). These undesirable side-reactions proceed, howEur. J. Clin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 3 ever, at a much slower rate. Consequently, by making spectrophotometric readings shortly after the addition of reagent, the specificity of bromcresol green assays for albumin is safeguarded, keeping it at a level http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Ektachem Slides Overestimate Serum Albumin in Patients with Nephrotic Syndrome

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1993.31.3.159
Publisher site
See Article on Publisher Site

Abstract

Introduction Bromcresol green and, to a lesser degree, bromcresol purple are widely used "chromogens" for the rapid colorimetric determination of albumin in serum (1--6). In spite of its lower specificity, bromcresol green has often been preferred over bromcresol purple in commercial reagents (7--9). Arguments against bromcresol purple include: 1) underestimation of albumin in cases of renal insufficiency (10) and obstructive jaundice (11); 2) lesser precision and sensitivity of bromcresol purple assays because of the 2 times lower molar absorptivity for the bromcresol purple-albumin dye complex as compared with bromcresol greenalbumin (9); 3) difficulties in the standardization and monitoring of bromcresol purple-based assays with non-human fluids, due to the fact that bromcresol purple -- unlike bromcresol green -- does not strongly bind to bovine serum albumin, often present in calibrators and quality control materials (9). On the other hand, bromcresol green suffers from a more pronounced reactivity with various globulins (5). These undesirable side-reactions proceed, howEur. J. Clin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 3 ever, at a much slower rate. Consequently, by making spectrophotometric readings shortly after the addition of reagent, the specificity of bromcresol green assays for albumin is safeguarded, keeping it at a level

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1993

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