Determination of the Catalytic Activity of Phospholipase A 2 : E. coli -Based Assay Compared to a Photometric Micelle Assay

Determination of the Catalytic Activity of Phospholipase A 2 : E. coli -Based Assay Compared to a... Introduction Various efforts have been made to develop sensitive assays for the determination of phospholipase A23) (1 -- 16). The choice of a detection method depends partly on the goal of a particular experiment. For example, some assays can be used on purified enzymes but are incompatible with crude systems, some methods provide a continuous assay and generate a time ) ^Zynies1. _ . ej ^ ,, . f ,TM A Phospholipase A2 = phosphatide 2-acylhydrolase (EC course while others do not, and some methods are amenable to automation while others are not. However, the most important consideration in the choice^ of the detection method is the sensitivity required for the particular enzyme. The required sensitivity depends on the quantity of enzyme available and on its specific activity. This point is especially important for the assay of non-pancreatic phospholipases A2 in human plasma, which are found in lower quantities and are, in general, less active than their counterparts from the pancreas or venom. ... lu"1"^ · . ,_ .j . «_ /TM Phospholipase Aj = phosphatide 1-acylhydrolase (EC 3.1.1.32)· Lipase = triacylglycerol aeylhydrolase (EC 3.1.1.3) The sensitivity of an. assay is influenced by a number , * t http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Determination of the Catalytic Activity of Phospholipase A 2 : E. coli -Based Assay Compared to a Photometric Micelle Assay

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1993.31.11.777
Publisher site
See Article on Publisher Site

Abstract

Introduction Various efforts have been made to develop sensitive assays for the determination of phospholipase A23) (1 -- 16). The choice of a detection method depends partly on the goal of a particular experiment. For example, some assays can be used on purified enzymes but are incompatible with crude systems, some methods provide a continuous assay and generate a time ) ^Zynies1. _ . ej ^ ,, . f ,TM A Phospholipase A2 = phosphatide 2-acylhydrolase (EC course while others do not, and some methods are amenable to automation while others are not. However, the most important consideration in the choice^ of the detection method is the sensitivity required for the particular enzyme. The required sensitivity depends on the quantity of enzyme available and on its specific activity. This point is especially important for the assay of non-pancreatic phospholipases A2 in human plasma, which are found in lower quantities and are, in general, less active than their counterparts from the pancreas or venom. ... lu"1"^ · . ,_ .j . «_ /TM Phospholipase Aj = phosphatide 1-acylhydrolase (EC 3.1.1.32)· Lipase = triacylglycerol aeylhydrolase (EC 3.1.1.3) The sensitivity of an. assay is influenced by a number , * t

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1993

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