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Determination of Free Fatty Acids: A Comparative Study of the Enzymatic Versus the Gas Chromatographic and the Colorimetric Method

Determination of Free Fatty Acids: A Comparative Study of the Enzymatic Versus the Gas... Mulder, Scheuten and Popp-Snijders: Determination of free fatty acids: A comparison of three methods J. Clin. Chem. Clin. Biochem. Vol. 21, 1983, pp. 823-827 Determination of Free Fatty Acids: A Comparative Study of the Enzymatic Versus the Gas Chromatographie and the Colorimetric Method By C Mulder, Department of Clinical Chemistry, Academic Hospital of the Free University, Amsterdam J. A. Schonten and Corrie Popp-Snijders Department of Internal Mediane, Academic Hospital of the Free University, Amsterdam (Received March 18/July 11, 1983) Summary: We determined the concentration of free fatty acids in 51 plasma samples by three different methods: a gas-liquid Chromatographie, a colorimetric and an enzymatic method. Values obtained by the three methods show a good correlation. The gas-liquid Chromatographie method was used äs the reference method. The results of the enzymatic method are about 5% too high and the colorimetric results have a bias of +27%. The coefficients of Variation of the three assays are 4, 7 and 5% respectively. Storage of the samples at --18 °C gives no change in the total amount of free fatty acids, enzymatically determined. Because of low costs (time, reagents and apparatus), combined with a sufficient accuracy and precision, the enzymatic method for http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Determination of Free Fatty Acids: A Comparative Study of the Enzymatic Versus the Gas Chromatographic and the Colorimetric Method

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References (8)

Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1983.21.12.823
Publisher site
See Article on Publisher Site

Abstract

Mulder, Scheuten and Popp-Snijders: Determination of free fatty acids: A comparison of three methods J. Clin. Chem. Clin. Biochem. Vol. 21, 1983, pp. 823-827 Determination of Free Fatty Acids: A Comparative Study of the Enzymatic Versus the Gas Chromatographie and the Colorimetric Method By C Mulder, Department of Clinical Chemistry, Academic Hospital of the Free University, Amsterdam J. A. Schonten and Corrie Popp-Snijders Department of Internal Mediane, Academic Hospital of the Free University, Amsterdam (Received March 18/July 11, 1983) Summary: We determined the concentration of free fatty acids in 51 plasma samples by three different methods: a gas-liquid Chromatographie, a colorimetric and an enzymatic method. Values obtained by the three methods show a good correlation. The gas-liquid Chromatographie method was used äs the reference method. The results of the enzymatic method are about 5% too high and the colorimetric results have a bias of +27%. The coefficients of Variation of the three assays are 4, 7 and 5% respectively. Storage of the samples at --18 °C gives no change in the total amount of free fatty acids, enzymatically determined. Because of low costs (time, reagents and apparatus), combined with a sufficient accuracy and precision, the enzymatic method for

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1983

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