Determination of Chondroitin-6-Sulphate by a Competitive Enzyme Immunoassay Using a Biotinylated Antigen

Determination of Chondroitin-6-Sulphate by a Competitive Enzyme Immunoassay Using a Biotinylated... Introduction Chondroitin sulphate consists of repeated disaccharide units containing N-acetyl-jD-galactosamine and Z)-glucuronic acid. This glycosaminoglycan is sulphated either on the C4 or C6 position of the amino sugar (1) and Eur. J. Clin. Chem. Clin. Biochem. / Vol. 32,1994 / No. 4 attached to a core protein to form proteoglycans. The macromolecule is synthesised in the Golgi apparatus, transported to the ceil surface and secreted into the extracellular space (2). Chondroitin sulphate is a major and ubiquitous component of the extracellular matrix of connective tissues (1). Several methods for the determi- nation of chondroitin sulphate have been described. These techniques use enzymatic digestion, precipitation and Chromatographie methods, especially high performance liquid chromatography (3,4). Methods have been reported recently, which use specific antibodies against different components of proteoglycans (e.g. keratan sulphate, dermatan sulphate) (5-8). So far, an enzyme immunoassay of chondroitin sulphate has not been described. The assay described here uses a monoclonal anti-chondroitin-6-sulphate antibody (9) and involves a competitive binding reaction between chondroitin-6-sulphate in the sample and biotinylated chondroitin-4-sulphate s labelled antigen. This enzyme immunoassay enables the determination of chondroitin6-sulphate in serum, urine and cell culture medium. K hnen et al.: Determination of chondroitin-6-sulphate peroxidase (60 U/l) were http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Determination of Chondroitin-6-Sulphate by a Competitive Enzyme Immunoassay Using a Biotinylated Antigen

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1994.32.4.293
Publisher site
See Article on Publisher Site

Abstract

Introduction Chondroitin sulphate consists of repeated disaccharide units containing N-acetyl-jD-galactosamine and Z)-glucuronic acid. This glycosaminoglycan is sulphated either on the C4 or C6 position of the amino sugar (1) and Eur. J. Clin. Chem. Clin. Biochem. / Vol. 32,1994 / No. 4 attached to a core protein to form proteoglycans. The macromolecule is synthesised in the Golgi apparatus, transported to the ceil surface and secreted into the extracellular space (2). Chondroitin sulphate is a major and ubiquitous component of the extracellular matrix of connective tissues (1). Several methods for the determi- nation of chondroitin sulphate have been described. These techniques use enzymatic digestion, precipitation and Chromatographie methods, especially high performance liquid chromatography (3,4). Methods have been reported recently, which use specific antibodies against different components of proteoglycans (e.g. keratan sulphate, dermatan sulphate) (5-8). So far, an enzyme immunoassay of chondroitin sulphate has not been described. The assay described here uses a monoclonal anti-chondroitin-6-sulphate antibody (9) and involves a competitive binding reaction between chondroitin-6-sulphate in the sample and biotinylated chondroitin-4-sulphate s labelled antigen. This enzyme immunoassay enables the determination of chondroitin6-sulphate in serum, urine and cell culture medium. K hnen et al.: Determination of chondroitin-6-sulphate peroxidase (60 U/l) were

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1994

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