Complex Analyte-Dependent and Analyte-Independent Interferences with Conjugated Bilirubin in the Enzymatic Phenol-Aminophenazone Peroxidase (PAP) Method for Creatinine Determination

Complex Analyte-Dependent and Analyte-Independent Interferences with Conjugated Bilirubin in the... Introduction Bilirubin is a well known negative interferent of the Jaffa method. There is some concern as to the degree of susceptibility for the phenol-aminophenazone peroxidase (PAP) method since compared with the Jaffe method in some studies (1, 2), the enzymatic assays for creatinine demonstrated less susceptibility to bilirubin and more as reported in others (3, 4). Moreover, published works suggest that conjugated bilirubin interferes more than unconjugated bilirubin in enzymatic assays (5, 6). Eur. J. Clin. Chem. Clin, Biochem. / Vol. 31,1993 / No. 12 , * r * j - - * j Our approach to interference studies is to derive a correlative equation by multiple regression analysis (7). The equation will contain terms which can be called analyte-dependent or analyte-independent. Analyte-independence is a term in which the degree of interference depends upon only the concentration of the interferent (8, 9). An example of this is pseudohyponatraemia is proportional only to the volume displacement caused by lipids. Another example is alkaline phosphatase, which when measured on the SMAC, is interfered by methotrexate because it ab- Eng et al.: Analyte-dcpendent/-indepcndent interferences by bilirubin on crcatinine PAP in biochemical measurements of patients. In this research, we studied the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Complex Analyte-Dependent and Analyte-Independent Interferences with Conjugated Bilirubin in the Enzymatic Phenol-Aminophenazone Peroxidase (PAP) Method for Creatinine Determination

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1993.31.12.839
Publisher site
See Article on Publisher Site

Abstract

Introduction Bilirubin is a well known negative interferent of the Jaffa method. There is some concern as to the degree of susceptibility for the phenol-aminophenazone peroxidase (PAP) method since compared with the Jaffe method in some studies (1, 2), the enzymatic assays for creatinine demonstrated less susceptibility to bilirubin and more as reported in others (3, 4). Moreover, published works suggest that conjugated bilirubin interferes more than unconjugated bilirubin in enzymatic assays (5, 6). Eur. J. Clin. Chem. Clin, Biochem. / Vol. 31,1993 / No. 12 , * r * j - - * j Our approach to interference studies is to derive a correlative equation by multiple regression analysis (7). The equation will contain terms which can be called analyte-dependent or analyte-independent. Analyte-independence is a term in which the degree of interference depends upon only the concentration of the interferent (8, 9). An example of this is pseudohyponatraemia is proportional only to the volume displacement caused by lipids. Another example is alkaline phosphatase, which when measured on the SMAC, is interfered by methotrexate because it ab- Eng et al.: Analyte-dcpendent/-indepcndent interferences by bilirubin on crcatinine PAP in biochemical measurements of patients. In this research, we studied the

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1993

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