Comparison of Four Grass Pollen Species Concerning Their Allergens of Grass Group V by 2D Immunoblotting and Microsequencing

Comparison of Four Grass Pollen Species Concerning Their Allergens of Grass Group V by 2D... Biol. Chem. Hoppe-Seyler Vol. 374, pp. 855-861, September 1993 Arnd PETERSEN, Gabriele SCHRAMM, Wolf-Meinhard BECKER and Max SCHLAAK Forschungsinstitut Borstel, Laborgruppe Allergologie, Borstel, Germany (Received 27 May /14 July 1993) Summary: The identication and characterization of allergenic components is a vital step towards improving diagnosis and therapy. Members of the grass family (Poaceae) reveal a high cross-reactivity among each other caused by the close phylogenetical relationship. In order to investigate the variability between allergenic components, we studied the allergen grass group V, one of the major allergens. Pollen extracts of 4 different tribes (timothy grass (Phlewn pratense) - Agrostidae, perennial rye grass (Lolium perenne) - Festuceae, meadow velvet (Holcus lanatus) - Aveneae, and rye (Secale cereale) Triticeae) of the Festucoideae subfamily were sepa- rated by 2D PAGE and investigated by immunoblotting using patients' poolserum and monoclonal antibodies (raised against group V allergens of timothy grass pollen). The antibodies identify different allergens in the four grass species.The components vary from 30-50 kDa and pi 4.8-7.0.The eight NH2-terminal amino acids were determined and indicated high similarities between the different components.These results cast doubt on the suitability of classifying allergens into groups based only on their molecular mass, isoelectric point and http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biological Chemistry Hoppe-Seyler de Gruyter

Comparison of Four Grass Pollen Species Concerning Their Allergens of Grass Group V by 2D Immunoblotting and Microsequencing

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Publisher
de Gruyter
Copyright
Copyright © 1993 by the
ISSN
0177-3593
eISSN
1437-4315
DOI
10.1515/bchm3.1993.374.7-12.855
Publisher site
See Article on Publisher Site

Abstract

Biol. Chem. Hoppe-Seyler Vol. 374, pp. 855-861, September 1993 Arnd PETERSEN, Gabriele SCHRAMM, Wolf-Meinhard BECKER and Max SCHLAAK Forschungsinstitut Borstel, Laborgruppe Allergologie, Borstel, Germany (Received 27 May /14 July 1993) Summary: The identication and characterization of allergenic components is a vital step towards improving diagnosis and therapy. Members of the grass family (Poaceae) reveal a high cross-reactivity among each other caused by the close phylogenetical relationship. In order to investigate the variability between allergenic components, we studied the allergen grass group V, one of the major allergens. Pollen extracts of 4 different tribes (timothy grass (Phlewn pratense) - Agrostidae, perennial rye grass (Lolium perenne) - Festuceae, meadow velvet (Holcus lanatus) - Aveneae, and rye (Secale cereale) Triticeae) of the Festucoideae subfamily were sepa- rated by 2D PAGE and investigated by immunoblotting using patients' poolserum and monoclonal antibodies (raised against group V allergens of timothy grass pollen). The antibodies identify different allergens in the four grass species.The components vary from 30-50 kDa and pi 4.8-7.0.The eight NH2-terminal amino acids were determined and indicated high similarities between the different components.These results cast doubt on the suitability of classifying allergens into groups based only on their molecular mass, isoelectric point and

Journal

Biological Chemistry Hoppe-Seylerde Gruyter

Published: Jan 1, 1993

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