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Assessment of Vitamin B 1 , B 2 and B 6 Status by Coenzyme Activation of Red Gell Enzymes Using a Centrifugal Analyser

Mak and Swaminathan: Assessment of vitamin BI, B2 and B6 status J. Clin. Chem. Clin. Biochem. Vol. 26, 1988, pp. 213-217 © 1988 Walter de Gruyter & Co. Berlin · New York Assessment of Vitamin BI, B2 and B6 Status by Coenzyme Activation of Red Cell Enzymes Using a Centrifugal Analyser By Y. T. Mak and R. Swaminathan Department of Chemical Pathology, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong (Received July 13, 1987/January 20, 1988) Summary: We describe a fully automated method for the assessment of vitamin B1? B2 and B6 status using a centrifugal analyser. The activation of the red cell enzymes transketolase, glutathione reductase and aspartate aminotransferase1) by their respective coenzymes were measured in freshly prepared haemolysate. The enzyme catalytic activities in the sample were measured with (maximal activity) and without (basal activity) the coenzyme, and the percentage activation was calculated. The between run precision for red cell transketolase, glutathione reductase and aspartate aminotransferase were 8.5%, 10.3% and 9.5% respectively. When whole blood was stored at room temperature for 6 hours, red cell aspartate aminotransferase activity significantly decreased (n = 10, p < 0.05). There were no significant changes in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

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