Introduction Enzyme immunoassays have been successfully employed for the quantitation of human autoantibodies of defined specificities using purified antigen adsorbed on a solid matrix (reviewed in I.e. (1)). Some variations of the procedure include the use of nylon beads as the solid support (2--4) instead of a conventional polystyrene surface, and the use of a fluorogenic substrate (5) for the evaluation of enzyme activity. In the present work, an enzyme-linked immunosorbent assay (ELISA) was evaluated as an alternative to the fluorescent antinuclear antibody (FANA) as*) List of antigens nDNA = native DNA UlsnRNP = ... small nuclear ribonucleoprotein Sm = an antigen, named from the initials of the patient who showed corresponding antibodies; a nuclear glycoprotein ANA = antinuclear antibodies RNP = Ribonucleoprotein SS-B/La = an antigen B, characterized by sera of certain patients with Sjögren syndrome (SS) SS-A/Ro = an antigen A, characterized by sera of certain patients with Sjögren syndrome (SS) Scl-70 = an antigen of Mr = 70.000, identified by sera of certain patients suffering from sclefoderma (Sc!) Eun J. Clin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 5 Materials and Methods Materials Polystyrene plates from Dynatech, USA, were used as the solid support
Clinical Chemistry and Laboratory Medicine – de Gruyter
Published: Jan 1, 1993
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