Assay of Antinuclear Antibodies by ELISA Using Nuclei as Antigen

Assay of Antinuclear Antibodies by ELISA Using Nuclei as Antigen Introduction Enzyme immunoassays have been successfully employed for the quantitation of human autoantibodies of defined specificities using purified antigen adsorbed on a solid matrix (reviewed in I.e. (1)). Some variations of the procedure include the use of nylon beads as the solid support (2--4) instead of a conventional polystyrene surface, and the use of a fluorogenic substrate (5) for the evaluation of enzyme activity. In the present work, an enzyme-linked immunosorbent assay (ELISA) was evaluated as an alternative to the fluorescent antinuclear antibody (FANA) as*) List of antigens nDNA = native DNA UlsnRNP = ... small nuclear ribonucleoprotein Sm = an antigen, named from the initials of the patient who showed corresponding antibodies; a nuclear glycoprotein ANA = antinuclear antibodies RNP = Ribonucleoprotein SS-B/La = an antigen B, characterized by sera of certain patients with Sjögren syndrome (SS) SS-A/Ro = an antigen A, characterized by sera of certain patients with Sjögren syndrome (SS) Scl-70 = an antigen of Mr = 70.000, identified by sera of certain patients suffering from sclefoderma (Sc!) Eun J. Clin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 5 Materials and Methods Materials Polystyrene plates from Dynatech, USA, were used as the solid support http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Clinical Chemistry and Laboratory Medicine de Gruyter

Assay of Antinuclear Antibodies by ELISA Using Nuclei as Antigen

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Publisher
de Gruyter
Copyright
Copyright © 2009 Walter de Gruyter
ISSN
1434-6621
eISSN
1437-4331
DOI
10.1515/cclm.1993.31.5.289
Publisher site
See Article on Publisher Site

Abstract

Introduction Enzyme immunoassays have been successfully employed for the quantitation of human autoantibodies of defined specificities using purified antigen adsorbed on a solid matrix (reviewed in I.e. (1)). Some variations of the procedure include the use of nylon beads as the solid support (2--4) instead of a conventional polystyrene surface, and the use of a fluorogenic substrate (5) for the evaluation of enzyme activity. In the present work, an enzyme-linked immunosorbent assay (ELISA) was evaluated as an alternative to the fluorescent antinuclear antibody (FANA) as*) List of antigens nDNA = native DNA UlsnRNP = ... small nuclear ribonucleoprotein Sm = an antigen, named from the initials of the patient who showed corresponding antibodies; a nuclear glycoprotein ANA = antinuclear antibodies RNP = Ribonucleoprotein SS-B/La = an antigen B, characterized by sera of certain patients with Sjögren syndrome (SS) SS-A/Ro = an antigen A, characterized by sera of certain patients with Sjögren syndrome (SS) Scl-70 = an antigen of Mr = 70.000, identified by sera of certain patients suffering from sclefoderma (Sc!) Eun J. Clin. Chem. Clin. Biochem. / Vol. 31,1993 / No. 5 Materials and Methods Materials Polystyrene plates from Dynatech, USA, were used as the solid support

Journal

Clinical Chemistry and Laboratory Medicinede Gruyter

Published: Jan 1, 1993

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